pET-32 Ek/LIC: Difference between revisions

Revision as of 22:17, 26 November 2007

At a Glance

Type	Cloning/Expression
Origin of Replication	pBR322
Copy #
Markers	Ampicillin
Link to Sequence
Features
Accession Number
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Notes

The pET-32 Ek/LIC vector is an Ek/LIC version of pET-32b(+). It is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag thioredoxin protein^[1], and His•Tag and STag coding sequences that are cleavable with enterokinase (Ek) protease.

History

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Sources

Novagen

References

↑ LaVallie, ER et al. (1993) A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm. Biotechnology (N.Y.) 11 187-93 PubMed

Sequence

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[PMID:7763371-1] LaVallie, ER et al. (1993) A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm. Biotechnology (N.Y.) 11 187-93 PubMed

[1]

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