pET-32 Ek/LIC: Difference between revisions
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==Notes== | ==Notes== | ||
The pET-32 Ek/LIC vector is an Ek/LIC version of pET-32b(+). It is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag thioredoxin protein, and His•Tag and STag coding sequences that are cleavable with enterokinase (Ek) protease. | The pET-32 Ek/LIC vector is an Ek/LIC version of pET-32b(+). It is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag thioredoxin protein<ref name='PMID:7763371'/>, and His•Tag and STag coding sequences that are cleavable with enterokinase (Ek) protease. | ||
==History {{HelpIcon|Help:Plasmid}}== | ==History {{HelpIcon|Help:Plasmid}}== | ||
Revision as of 22:17, 26 November 2007
At a Glance
| Type |
Cloning/Expression |
|---|---|
| Origin of Replication |
pBR322 |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features | |
| Accession Number | |
| edit table | |
Notes
The pET-32 Ek/LIC vector is an Ek/LIC version of pET-32b(+). It is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag thioredoxin protein[1], and His•Tag and STag coding sequences that are cleavable with enterokinase (Ek) protease.
History
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Sources
References
- ↑ LaVallie, ER et al. (1993) A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm. Biotechnology (N.Y.) 11 187-93 PubMed
- ↑ Bi, X et al. (2005) The rice nucellin gene ortholog OsAsp1 encodes an active aspartic protease without a plant-specific insert and is strongly expressed in early embryo. Plant Cell Physiol. 46 87-98 PubMed
- ↑ Mehenni, H et al. (2005) LKB1 interacts with and phosphorylates PTEN: a functional link between two proteins involved in cancer predisposing syndromes. Hum. Mol. Genet. 14 2209-19 PubMed
- ↑ McDowell, JV et al. (2004) Demonstration of the involvement of outer surface protein E coiled coil structural domains and higher order structural elements in the binding of infection-induced antibody and the complement-regulatory protein, factor H. J. Immunol. 173 7471-80 PubMed
Sequence
You can help EcoliWiki by providing database accessions or, if none are available, the sequence of this plasmid.