dcm:Quickview: Difference between revisions
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===Notes=== | ===Notes=== | ||
Method of Mutagenesis: Alkylating agent (N-methyl-N-nitro-nitrosoguanidine)<ref name=PMID:4576399/> | '''Method of Mutagenesis:''' Log-phase cultures of strain GM 1 were treated with Alkylating agent (N-methyl-N-nitro-nitrosoguanidine)<ref name=PMID:4576399/> | ||
Phenotype Observed: reduced or absent activity, | '''Phenotype Observed:''' Mutants were tested for S-adenosylmethionine methyltransferase activity by measuring the levels of DNA-cytosine (S-adenosylmethionine and DNA-adenine. The mutants showed reduced or absent activity. Also, deficient in methylcytosine in DNA. <ref name=PMID:4576399/> | ||
Revision as of 15:54, 8 November 2007
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| References | Suggestions |
| Gene Name | |
|---|---|
| Product(s) |
Dcm product[1]: DNA cytosine methylase product, B1961 product, Mec product |
| Description | |
| Knock-Out Phenotype | |
| Regulation/Expression | |
| Regulation/Activity | |
| edit table | |
See Help:Quickview for help with entering information in the Quickview table.
Notes
Method of Mutagenesis: Log-phase cultures of strain GM 1 were treated with Alkylating agent (N-methyl-N-nitro-nitrosoguanidine)[4]
Phenotype Observed: Mutants were tested for S-adenosylmethionine methyltransferase activity by measuring the levels of DNA-cytosine (S-adenosylmethionine and DNA-adenine. The mutants showed reduced or absent activity. Also, deficient in methylcytosine in DNA. [4]
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 Marinus, MG & Morris, NR (1973) Isolation of deoxyribonucleic acid methylase mutants of Escherichia coli K-12. J. Bacteriol. 114 1143-50 PubMed