WK6mutS(λ): Difference between revisions

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| [https://bccm.belspo.be/catalogues/genecorner-host-details?NUM=LMBP%201466 LMBP 1466]  
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/388 LMBP 1466]  
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| References
| References

Latest revision as of 11:33, 28 October 2024

Name WK6mutS(λ)
Synonyms
Genotype F' lacIq ∆(lacZ)M15 proA+B+ traD36I ∆(lac-proB) galE rpsL mutS::Tn10 λ
Phenotype Streptomycin resistant, tetracyclin resistant
Sources LMBP 1466
References PMID:3038536 PMID:2501754

Notes

Useful strain for site-directed mutagenesis; disabled mismatch repair due to knocked-out mutS.
The Tn10 insertion renders the strain tetR.
Not to be used as permanent storage host for plasmids because of the danger of accumulating spontaneous mutations.