MG1655 seqA-PAmCherry: Difference between revisions
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| [ | | [https://bccm.belspo.be/catalogues/genecorner-host-details?NUM=LMBP%209778 LMBP 9778] | ||
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| References | | References | ||
Revision as of 12:29, 16 October 2020
| Name | MG1655 seqA-PAmCherry |
| Synonyms | |
| Genotype | F– λ– ilvG– rfb-50 rph-1 seqA-PAmCherry:kmR |
| Phenotype | Kanamycin resistant |
| Sources | LMBP 9778 |
| References | PMID:26449690 |
Notes
The native seqA gene was fused at its 3' end to the fluorescent protein gene PAmCherry, separated with a linker (ASPPPGRSR) and followed by a kanamycin resistance marker. The entire cassette was integrated in the chromosome.
PAmCherry is a photoactivatable variant of the red fluorescent protein mCherry. PAmCherry is not fluorescent until it is exposed to 350-400 nm light.
This strain can be used to study the subcellular localization of the E. coli DNA binding protein SeqA.