HB101: Difference between revisions

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{{Strain Help}}
{| class="wikitable" border="1"
|-
| Name
| KM5219
|-
| Synonyms
|
|-
| Genotype
| Different sources have different genotypes for HB101. The genotype listed below is from the Coli Genetics Stock Center.
| F<sup>-</sup> Lambda<sup>-</sup> araC14 leuB6(Am) DE(gpt-proA)62 lacY1 glnX44(AS) galK2(Oc) recA13 rpsL20(strR) xylA5 mtl-1 thiE1 hsdS20(r<sub>B</sub><sup>-</sup>, m<sub>B</sub><sup>-</sup>)
|
| NEB: includes DE(mcrC-mrr)
| Promega (and others): proA2
|-
| Phenotype
| Streptomycin resistance
| Lacks the K-12 restriction-modification system
|-
| Sources
| [https://bccm.belspo.be/catalogues/genecorner-host-details?NUM=LMBP%20684 LMBP 684]
| [http://cgsc.biology.yale.edu/Strain.php?ID=130465 CGSC 12554
|-
| References
| Boyer, H.W. and Roulland-Dussoix, D. (1969) J. Mol. Biol. 41, 459.
| Smith, M., Lorow, D., and Jessee, J. (1989) FOCUS 11, 56.
| Lacks S and Greenberg JR (1977) J Mol Biol 114:153.
|}


===Notes===


==Alternate Names ==


==Sources ==
 
[[Category:BCCM/GeneCorner]][[Category:Strains]]
==Chromosomal Genotype==
Different sources have different genotypes for HB101. The genotype listed below is from the Coli Genetics Stock Center.
 
F<sup>-</sup> Lambda<sup>-</sup> araC14 leuB6(Am) DE(gpt-proA)62 lacY1 glnX44(AS) galK2(Oc) recA13 rpsL20(strR) xylA5 mtl-1 thiE1 hsdS20(r<sub>B</sub><sup>-</sup>, m<sub>B</sub><sup>-</sup>)<ref>http://cgsc.biology.yale.edu/Strain.php?ID=130465</ref>
 
NEB: includes DE(mcrC-mrr)
Promega (and others): proA2
 
==Plasmids ==
 
==Phage ==
 
==Phenotypes ==
Streptomycin resistance
 
Lacks the K-12 restriction-modification system
 
==Construction ==
 
==Notes==
 
Boyer, H.W. and Roulland-Dussoix, D. (1969) J. Mol. Biol. 41, 459.
 
Smith, M., Lorow, D., and Jessee, J. (1989) FOCUS 11, 56.
 
Lacks S and Greenberg JR (1977) J Mol Biol 114:153.
 
==References==
{{RefHelp}}
<references/>
 
__NOTOC__
[[Category:Strains]]

Revision as of 12:15, 16 October 2020

Name KM5219
Synonyms
Genotype Different sources have different genotypes for HB101. The genotype listed below is from the Coli Genetics Stock Center. F- Lambda- araC14 leuB6(Am) DE(gpt-proA)62 lacY1 glnX44(AS) galK2(Oc) recA13 rpsL20(strR) xylA5 mtl-1 thiE1 hsdS20(rB-, mB-) NEB: includes DE(mcrC-mrr) Promega (and others): proA2
Phenotype Streptomycin resistance Lacks the K-12 restriction-modification system
Sources LMBP 684 [http://cgsc.biology.yale.edu/Strain.php?ID=130465 CGSC 12554
References Boyer, H.W. and Roulland-Dussoix, D. (1969) J. Mol. Biol. 41, 459. Smith, M., Lorow, D., and Jessee, J. (1989) FOCUS 11, 56. Lacks S and Greenberg JR (1977) J Mol Biol 114:153.

Notes

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