SG4121: Difference between revisions
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| [ | | [https://bccm.belspo.be/catalogues/genecorner-host-details?NUM=LMBP%20671 LMBP 671] | ||
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| References | | References | ||
Revision as of 12:06, 16 October 2020
| Name | SG4121 |
| Synonyms | |
| Genotype | lon-100 Δ(gal-pgl)324 lac thi rpsL recA hsdR |
| Phenotype | Streptomycin resistant, non-mucoid, Lon- |
| Sources | LMBP 671 |
| References | PMID:1312934 |
Notes
The Lon protease plays a major role in the proteolytic machinery of E. coli. Lon mutants are highly pleioptropic and produce mucoid colonies that are difficult to handle. The (gal-pgl) deletion eliminates mucoidy and restores normal colony morphology.
Lon mutants may be useful in stabilizing heterologous proteins expressed in E. coli (see e.g. Remaut et al., Nucleic Acids Res. 11 (1983), 4677-4688).
In addition, this strain is K12 restriction negative / modification positive, and RecA deficient.