WK6mutS(λ): Difference between revisions
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| [ | | [https://bccm.belspo.be/catalogues/genecorner-host-details?NUM=LMBP%201466 LMBP 1466] | ||
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| References | | References | ||
Revision as of 11:30, 16 October 2020
| Name | WK6mutS(λ) |
| Synonyms | |
| Genotype | F' lacIq ∆(lacZ)M15 proA+B+ traD36I ∆(lac-proB) galE rpsL mutS::Tn10 λ |
| Phenotype | Streptomycin resistant, tetracyclin resistant |
| Sources | LMBP 1466 |
| References | PMID:3038536 PMID:2501754 |
Notes
Useful strain for site-directed mutagenesis; disabled mismatch repair due to knocked-out mutS.
The Tn10 insertion renders the strain tetR.
Not to be used as permanent storage host for plasmids because of the danger of accumulating spontaneous mutations.