M5219: Difference between revisions
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| [ | | [https://bccm.belspo.be/catalogues/genecorner-host-details?NUM=LMBP%20130 LMBP 130] | ||
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| References | | References | ||
Revision as of 11:23, 16 October 2020
| Name | KM5219 |
| Synonyms | |
| Genotype | lacZam trpAam rpsL lambda(bio252 cI857 ΔH1) |
| Phenotype | streptomycin resistant |
| Sources | LMBP 130 |
| References | PMID:6271633 |
Notes
Expression host for plasmids containing phage lambda's PL or PR promoter. Induction is obtained by shifting the culture from 28°C to 42°C. The resident lambda is a defective lysogen. ΔH1 removes part of cro and all genes to the right of it. bio252 removes all genes to the left of cIII. cI857 is ind-. At 42°C, N is expressed from the lysogen. As a consequence, transcription termination signals that might be present downstream from the PL promoter and the nutL site, as present on the pPL-series of plasmids, can be overridden. Useful for the expression of cloned genes carrying transcription terminators between the start of the gene and the insertion point.