M5219: Difference between revisions
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Created page with "{| class="wikitable" border="1" |- | Name | KM5219 |- | Synonyms | |- | Genotype | lacZam trpAam rpsL lambda(bio252 cI857 ΔH1) |- | Phenotype | streptomycin resistant |- | S..." |
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| Sources | | Sources | ||
| [http://bccm.belspo.be/catalogues/files/ | | [http://bccm.belspo.be/catalogues/files/genecorner-hosts/M5219.pdf LMBP 69] | ||
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| References | | References | ||
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[[Category:BCCM/ | [[Category:BCCM/GeneCorner]][[Category:Strains]] | ||
Revision as of 13:24, 16 August 2018
| Name | KM5219 |
| Synonyms | |
| Genotype | lacZam trpAam rpsL lambda(bio252 cI857 ΔH1) |
| Phenotype | streptomycin resistant |
| Sources | LMBP 69 |
| References | PMID:6271633 |
Notes
Expression host for plasmids containing phage lambda's PL or PR promoter. Induction is obtained by shifting the culture from 28°C to 42°C. The resident lambda is a defective lysogen. ΔH1 removes part of cro and all genes to the right of it. bio252 removes all genes to the left of cIII. cI857 is ind-. At 42°C, N is expressed from the lysogen. As a consequence, transcription termination signals that might be present downstream from the PL promoter and the nutL site, as present on the pPL-series of plasmids, can be overridden. Useful for the expression of cloned genes carrying transcription terminators between the start of the gene and the insertion point.