MC1061Rif: Difference between revisions
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Created page with "{| class="wikitable" border="1" |- | Name | MC1061Rif |- | Synonyms | |- | Genotype | ∆(araA-leu)7697 [araD139]<sub>B/r</sub> ∆(codB-lacI)3 = ∆lac74 galK16 galE15 e14- ..." |
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| Sources | | Sources | ||
| [http://bccm.belspo.be/catalogues/files/ | | [http://bccm.belspo.be/catalogues/files/genecorner-hosts/MC1061Rif.pdf LMBP 3319] | ||
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| References | | References | ||
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[[Category:BCCM/ | [[Category:BCCM/GeneCorner]][[Category:Strains]] | ||
Revision as of 13:23, 16 August 2018
| Name | MC1061Rif |
| Synonyms | |
| Genotype | ∆(araA-leu)7697 [araD139]B/r ∆(codB-lacI)3 = ∆lac74 galK16 galE15 e14- mcrA0 relA1 rpsL150 spoT1 mcrB1 hsdR2 λ+ |
| Phenotype | rifampicin resistant |
| Sources | LMBP 3319 |
| References | PMID:6997493 |
Notes
Useful host for primary transformation. Transforms very well by the CaCl2 method (107/µg). There is no Type I restriction; incoming DNA receives the E. coli K modification.
Lysogenized with λ wild type.
As this strain is deleted for the lacI repressor gene, it is not a suitable host for plasmids carrying the lac promoter or derivatives thereof, such as tac, trc, N25/O2 … promoters. In the absence of repression, continuous transcription from the lac promoter is likely to result in plasmid instability.