MG1655 seqA-mEOS3.2: Difference between revisions
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Created page with "{| class="wikitable" border="1" |- | Name | MG1655 seqA-mEOS3.2 |- | Synonyms | |- | Genotype | F<sup>–</sup> λ<sup>–</sup> ilvG<sup>–</sup> rfb-50 rph-1 seqA-mEOS3.2:..." |
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| Sources | | Sources | ||
| [http://bccm.belspo.be/catalogues/files/ | | [http://bccm.belspo.be/catalogues/files/genecorner-hosts/MG1655%20seqA-mEOS3-2.pdf LMBP 9777] | ||
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| References | | References | ||
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[[Category:BCCM/ | [[Category:BCCM/GeneCorner]][[Category:Strains]] | ||
Revision as of 13:22, 16 August 2018
| Name | MG1655 seqA-mEOS3.2 |
| Synonyms | |
| Genotype | F– λ– ilvG– rfb-50 rph-1 seqA-mEOS3.2:kmR |
| Phenotype | Kanamycin resistant |
| Sources | LMBP 9777 |
| References | PMID:26449690 |
Notes
The native seqA gene was fused at its 3' end to the fluorescent protein gene mEOS3.2, separated with a linker (ASPPPGRSR) and followed by a kanamycin resistance marker. The entire cassette was integrated in the chromosome.
This strain can be used to study the subcellular localization of the E. coli DNA binding protein SeqA.