K12ΔH1Δtrp: Difference between revisions

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Created page with "{| class="wikitable" border="1" |- | Name | K12ΔH1Δtrp |- | Synonyms | K12deltaH1deltatrp |- | Genotype | lacZam Δ(bio-uvrB) Δ(trpE-A)2 rpsL Δ(Nam7Nam53 cI857 ΔH1) |- | ..."
 
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| Sources
| Sources
| [http://bccm.belspo.be/catalogues/files/lmbp-hosts/K12deltaH1deltatrp.pdf LMBP 69]
| [http://bccm.belspo.be/catalogues/files/lmbp-hosts/k12deltah1deltatrp.pdf LMBP 69]
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| References
| References

Revision as of 09:39, 4 July 2016

Name K12ΔH1Δtrp
Synonyms K12deltaH1deltatrp
Genotype lacZam Δ(bio-uvrB) Δ(trpE-A)2 rpsL Δ(Nam7Nam53 cI857 ΔH1)
Phenotype streptomycin resistant
Sources LMBP 69
References PMID:372049 PMID:6271633

Notes

Expression host for plasmids containing phage lambda's PL or PR promoter. Induction is obtained by shifting the culture from 28°C to 42°C. The resident lambda is a defective lysogen. ΔH1 removes part of cro and all genes to the right of it. cI857 is ind-.