PMID:9515930: Difference between revisions
New PMID: Page! |
Fill PMID: Page! |
||
| Line 1: | Line 1: | ||
{{RightTOC}} | |||
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.184458.R570ea57c32699--> | |||
<!-- | |||
****************************************************************************************** | |||
* | |||
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | |||
* | |||
****************************************************************************************** --> | |||
{| id="R570ea57c32699" class=" tableEdit PMID_info_table" | |||
|- | |||
!align=left |Citation | |||
|| | |||
'''Leffers, GG Jr and Gottesman, S''' (1998) Lambda Xis degradation in vivo by Lon and FtsH. ''J. Bacteriol.'' '''180''':1573-7 | |||
|- | |||
!align=left |Abstract | |||
|| | |||
Lambda Xis, which is required for site-specific excision of phage lambda from the bacterial chromosome, has a much shorter functional half-life than Int, which is required for both integration and excision (R. A. Weisberg and M. E. Gottesman, p. 489-500, in A. D. Hershey, ed., The Bacteriophage Lambda, 1971). We found that Xis is degraded in vivo by two ATP-dependent proteases, Lon and FtsH (HflB). Xis was stabilized two- to threefold more than in the wild type in a lon mutant and as much as sixfold more in a lon ftsH double mutant at the nonpermissive temperature for the ftsH mutation. Integration of lambda into the bacterial chromosome was delayed in the lon ftsH background, suggesting that accumulation of Xis in vivo interferes with integration. Overexpression of Xis in wild-type cells from a multicopy plasmid inhibited integration of lambda and promoted curing of established lysogens, confirming that accumulation of Xis interferes with the ability of Int to establish and maintain an integrated prophage. | |||
|- | |||
!align=left |Links | |||
|| | |||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9515930 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC107061 PMC107061] | |||
|- | |||
!align=left |Keywords | |||
|| | |||
ATP-Dependent Proteases; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacteriophage lambda/genetics; Bacteriophage lambda/metabolism; DNA Nucleotidyltransferases/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/virology; Escherichia coli Proteins; Heat-Shock Proteins/genetics; Heat-Shock Proteins/metabolism; Lysogeny/genetics; Membrane Proteins/genetics; Membrane Proteins/metabolism; Protease La; Serine Endopeptidases/genetics; Serine Endopeptidases/metabolism; Viral Proteins | |||
|- class="tableEdit_footer" | |||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.184458.R570ea57c32699&page=184458&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> || | |||
|} | |||
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.184458.R570ea57c32699--> | |||
==Significance {{HelpIcon|Help:Literature}} == | |||
{{LitSignificance}} | |||
== Useful Materials and Methods {{HelpIcon|Help:Literature}}== | |||
{{LitMaterials}} | |||
==Annotations {{HelpIcon|Help:Literature}}== | |||
<annotationlinks/> | |||
==EcoliWiki Links {{HelpIcon|Help:Literature}} == | |||
{{LitLinks}} | |||
==See also== | |||
==References== | |||
{{RefHelp}} | |||
<references/> | |||
Latest revision as of 20:01, 13 April 2016
| Citation |
Leffers, GG Jr and Gottesman, S (1998) Lambda Xis degradation in vivo by Lon and FtsH. J. Bacteriol. 180:1573-7 |
|---|---|
| Abstract |
Lambda Xis, which is required for site-specific excision of phage lambda from the bacterial chromosome, has a much shorter functional half-life than Int, which is required for both integration and excision (R. A. Weisberg and M. E. Gottesman, p. 489-500, in A. D. Hershey, ed., The Bacteriophage Lambda, 1971). We found that Xis is degraded in vivo by two ATP-dependent proteases, Lon and FtsH (HflB). Xis was stabilized two- to threefold more than in the wild type in a lon mutant and as much as sixfold more in a lon ftsH double mutant at the nonpermissive temperature for the ftsH mutation. Integration of lambda into the bacterial chromosome was delayed in the lon ftsH background, suggesting that accumulation of Xis in vivo interferes with integration. Overexpression of Xis in wild-type cells from a multicopy plasmid inhibited integration of lambda and promoted curing of established lysogens, confirming that accumulation of Xis interferes with the ability of Int to establish and maintain an integrated prophage. |
| Links | |
| Keywords |
ATP-Dependent Proteases; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacteriophage lambda/genetics; Bacteriophage lambda/metabolism; DNA Nucleotidyltransferases/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/virology; Escherichia coli Proteins; Heat-Shock Proteins/genetics; Heat-Shock Proteins/metabolism; Lysogeny/genetics; Membrane Proteins/genetics; Membrane Proteins/metabolism; Protease La; Serine Endopeptidases/genetics; Serine Endopeptidases/metabolism; Viral Proteins |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.