PMID:3311747

Citation	Westh Hansen, SE, Jensen, N and Munch-Petersen, A (1987) Studies on the sequence and structure of the Escherichia coli K-12 nupG gene, encoding a nucleoside-transport system. Eur. J. Biochem. 168:385-91
Abstract	The nupG gene, encoding one of the two active nucleoside-transport systems in Escherichia coli K-12, has been cloned on the multicopy plasmid pBR322 and derivatives thereof. The recombinant plasmids complemented a chromosomal nupG mutation. A genetic map was determined by digestion with restriction endonucleases and the nucleotide sequence of a 3-kb stretch of DNA has been determined on fragments cloned into M13 phages. An open reading frame of 1254 bp, encoding a protein with a calculated molecular mass of 45.333 kDa, was deduced to be the coding region of nupG. Minicell-forming strains carrying plasmids containing this gene were shown to produce a hydrophobic, membrane-bound polypeptide with an apparent molecular mass of approximately 43 kDa.
Links	PubMed
Keywords	Base Sequence; Binding Sites; Biological Transport, Active; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Escherichia coli/genetics; Escherichia coli/metabolism; Genes; Molecular Sequence Data; Nucleosides/metabolism; Peptide Biosynthesis; Peptides/genetics; Plasmids; Promoter Regions, Genetic
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