PMID:785220: Difference between revisions

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<span style = 'font-size: large'> '''Borck, K, Beggs, JD, Brammar, WJ, Hopkins, AS and Murray, NE'''  (1976) The construction in vitro of transducing derivatives of phage lambda.''Mol. Gen. Genet.'' '''146''':199-207</span>
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!align=left  |Citation
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'''No author listed'''  (1976) The construction in vitro of transducing derivatives of phage lambda. ''Mol. Gen. Genet.'' '''146''':199-207
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!align=left  |Abstract
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Methods are described for the construction of plaque-forming, transducing derivatives of phage lambda, using appropirate receptor genomes and fragments of DNA generated by the restriction enzymes endo R.EcoRI and endo R.HindIII. The general properties of the transducing derivatives are described and discussed. Plaque-forming phages carrying the E. coli trp, his, cysB, thyA, supD, supE, sup F, hsd, tna and lig genes have been isolated.
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!align=left  |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=785220 PubMed]


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|{{TableHeadingColor}} |'''Abstract''' || Methods are described for the construction of plaque-forming, transducing derivatives of phage lambda, using appropirate receptor genomes and fragments of DNA generated by the restriction enzymes endo R.EcoRI and endo R.HindIII. The general properties of the transducing derivatives are described and discussed. Plaque-forming phages carrying the E. coli trp, his, cysB, thyA, supD, supE, sup F, hsd, tna and lig genes have been isolated.
!align=left  |Keywords
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Chromosomes, Bacterial; Coliphages/metabolism; DNA, Bacterial; DNA, Viral; Endonucleases/metabolism; Escherichia coli/enzymology; Genetic Techniques; Transduction, Genetic
|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=785220 PubMed]
 
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==Significance {{HelpIcon|Help:Literature}} ==
==Significance {{HelpIcon|Help:Literature}} ==
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Revision as of 19:39, 1 September 2013

Citation

No author listed (1976) The construction in vitro of transducing derivatives of phage lambda. Mol. Gen. Genet. 146:199-207

Abstract

Methods are described for the construction of plaque-forming, transducing derivatives of phage lambda, using appropirate receptor genomes and fragments of DNA generated by the restriction enzymes endo R.EcoRI and endo R.HindIII. The general properties of the transducing derivatives are described and discussed. Plaque-forming phages carrying the E. coli trp, his, cysB, thyA, supD, supE, sup F, hsd, tna and lig genes have been isolated.

Links

PubMed

Keywords

Chromosomes, Bacterial; Coliphages/metabolism; DNA, Bacterial; DNA, Viral; Endonucleases/metabolism; Escherichia coli/enzymology; Genetic Techniques; Transduction, Genetic

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