PMID:6267426: Difference between revisions

Revision as of 19:31, 1 September 2013

Citation	No author listed (1981) Cloning, restriction endonuclease mapping and post-transcriptional regulation of rpsA, the structural gene for ribosomal protein S1. Mol. Gen. Genet. 181:548-51
Abstract	Transducing lambda phages have been isolated that carry segments of the Escherichia coli chromosome in the aspC region, 20.5 min on the E. coli map. One of these phages, lambda aspC2, carries rpsA, the structural gene for the ribosomal protein S1. A three kilobase fragment from this phage, cloned into either the plasmid pACYC184 or the plasmid pBR322, was found to express S1. In cells carrying the rpsA gene on the high copy number plasmid pBR322 the rate of rpsA mRNA synthesis was increased 40-fold, whereas the rate of protein S1 synthesis was doubled, in comparison with these rates in an rpsA haploid.
Links	PubMed
Keywords	Cloning, Molecular; DNA Restriction Enzymes/metabolism; Escherichia coli/genetics; Gene Expression Regulation; Genes; Plasmids; Protein Biosynthesis; Ribosomal Proteins/genetics; Transcription, Genetic
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