PMID:3486673: Difference between revisions

Revision as of 19:25, 1 September 2013

Citation	No author listed (1986) Transition-state stabilization by adenosine deaminase: structural studies of its inhibitory complex with deoxycoformycin. Biochemistry 25:1616-21
Abstract	Experiments with radioactive deoxycoformycin indicate that the inhibitor is released from calf intestinal adenosine deaminase after the enzyme-inhibitor complex is disrupted by denaturation. Experiments with 2H2O and H218O indicate that the enzyme does not catalyze elimination-addition reactions that could have led to reversible covalent derivatization of the enzyme. Ultraviolet difference spectra and the influence of pH on inhibitor binding indicate that deoxycoformycin is bound intact as the neutral species, at a binding site that is less polar than solvent water. The enzyme-inhibitor complex appears to be held together by hydrogen bonds of extraordinary stability (ca. 10 kcal/mol). These results suggest that deamination proceeds by direct water attack, the enzyme acting as a general-base catalyst.
Links	PubMed
Keywords	Adenosine Deaminase Inhibitors; Animals; Cattle; Coformycin/analogs & derivatives; Coformycin/pharmacology; Drug Stability; Hydrogen-Ion Concentration; Intestines/enzymology; Kinetics; Ligands; Nucleoside Deaminases/antagonists & inhibitors; Pentostatin; Protein Binding; Ribonucleosides/pharmacology; Spectrophotometry, Ultraviolet
edit table

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

@@ Line 38: / Line 38: @@
 ==Annotations {{HelpIcon|Help:Literature}}==
-<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.148920.E490f69a77d984-->
+<protect><annotationlinks/></protect>
-<!--
-******************************************************************************************
-*
-*   ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
-*
-****************************************************************************************** -->
-{|   id="E490f69a77d984"  class=" tableEdit GO_table_reference"
-|- {{table heading style}}
-!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
-|-
-|
-Add
-|
-|
-GO:0005488
-|
-binding
-|
-IDA: Inferred from Direct Assay
-|
-CHEBI:27834
-|
-F
-|
-Add activity is inhibited by deoxycoformycin
-|
-complete
-|- class="tableEdit_footer"
-|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.148920.E490f69a77d984&page=148920&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table]</span> || || || || || || || ||
-|}
-<!--box uid=63ce1649aab8b3180235b0af7618f667.148920.E490f69a77d984--></protect>
 ==EcoliWiki Links {{HelpIcon|Help:Literature}} ==