PMID:2990907: Difference between revisions

Revision as of 19:21, 1 September 2013

Citation	No author listed (1985) Sequence of the relB transcription unit from Escherichia coli and identification of the relB gene. EMBO J. 4:1059-66
Abstract	Escherichia coli relB mutants react to amino acid starvation by several abnormal responses, including accumulation of a translational inhibitor. We have isolated a relB-complementing plasmid from the Clarke and Carbon E. coli DNA library. From this plasmid we sequenced a 2140-bp segment which included the relB gene by the following two criteria: (i) it complements chromosomal relB mutations, (ii) the corresponding DNA segment cloned from chromosomal DNA of three relB mutants was defective in relB complementation. All three mutations fell within an open reading frame of 79 amino acids. A polypeptide of 9 kd compatible with this open reading frame was synthesized in maxicells and is in all probability the product of the relB gene. By nuclease S1 mapping we have determined the transcription start and stop of an 870 base transcript of the relB gene.
Links	PubMed PMC554300
Keywords	Amino Acid Sequence; Bacterial Proteins/genetics; Base Sequence; Chromosome Mapping; Chromosomes, Bacterial; Escherichia coli/genetics; Genes; Genes, Bacterial; Genotype; Plasmids; Species Specificity; Transcription, Genetic
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