PMID:21421753: Difference between revisions
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Revision as of 19:12, 1 September 2013
| Citation |
No author listed (2011) Efficient extension of slipped DNA intermediates by DinB is required to escape primer template realignment by DnaQ. J. Bacteriol. 193:2637-41 |
|---|---|
| Abstract |
We show that Escherichia coli DinB polymerase, which creates single-base deletions, prefers to extend slipped DNA substrates with the skipped base at the -4 position. A DinB(Y79L) variant, which extends these substrates less efficiently in vitro, allows the proofreading function of polymerase III to reverse their formation in vivo. |
| Links |
PubMed PMC3133156 Online version:10.1128/JB.00005-11 |
| Keywords |
Amino Acid Substitution/genetics; DNA Polymerase III/metabolism; DNA Replication; DNA, Bacterial/biosynthesis; Escherichia coli/enzymology; Escherichia coli/physiology; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Models, Biological; Mutant Proteins/genetics; Mutant Proteins/metabolism; Mutation, Missense; Sequence Deletion |
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