PMID:17483465: Difference between revisions

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<span style = 'font-size: large'> '''Rubio, MA, Pastar, I, Gaston, KW, Ragone, FL, Janzen, CJ, Cross, GA, Papavasiliou, FN and Alfonzo, JD'''  (2007) An adenosine-to-inosine tRNA-editing enzyme that can perform C-to-U deamination of DNA.''Proc. Natl. Acad. Sci. U.S.A.'' '''104''':7821-6</span>
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|{{TableHeadingColor}} |'''Abstract''' || Adenosine-to-inosine editing in the anticodon of tRNAs is essential for viability. Enzymes mediating tRNA adenosine deamination in bacteria and yeast contain cytidine deaminase-conserved motifs, suggesting an evolutionary link between the two reactions. In trypanosomatids, tRNAs undergo both cytidine-to-uridine and adenosine-to-inosine editing, but the relationship between the two reactions is unclear. Here we show that down-regulation of the Trypanosoma brucei tRNA-editing enzyme by RNAi leads to a reduction in both C-to-U and A-to-I editing of tRNA in vivo. Surprisingly, in vitro, this enzyme can mediate A-to-I editing of tRNA and C-to-U deamination of ssDNA but not both in either substrate. The ability to use both DNA and RNA provides a model for a multispecificity editing enzyme. Notably, the ability of a single enzyme to perform two different deamination reactions also suggests that this enzyme still maintains specificities that would have been found in the ancestor deaminase, providing a first line of evidence for the evolution of editing deaminases.
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'''No author listed'''  (2007) An adenosine-to-inosine tRNA-editing enzyme that can perform C-to-U deamination of DNA. ''Proc. Natl. Acad. Sci. U.S.A.'' '''104''':7821-6
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!align=left  |Abstract
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Adenosine-to-inosine editing in the anticodon of tRNAs is essential for viability. Enzymes mediating tRNA adenosine deamination in bacteria and yeast contain cytidine deaminase-conserved motifs, suggesting an evolutionary link between the two reactions. In trypanosomatids, tRNAs undergo both cytidine-to-uridine and adenosine-to-inosine editing, but the relationship between the two reactions is unclear. Here we show that down-regulation of the Trypanosoma brucei tRNA-editing enzyme by RNAi leads to a reduction in both C-to-U and A-to-I editing of tRNA in vivo. Surprisingly, in vitro, this enzyme can mediate A-to-I editing of tRNA and C-to-U deamination of ssDNA but not both in either substrate. The ability to use both DNA and RNA provides a model for a multispecificity editing enzyme. Notably, the ability of a single enzyme to perform two different deamination reactions also suggests that this enzyme still maintains specificities that would have been found in the ancestor deaminase, providing a first line of evidence for the evolution of editing deaminases.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=17483465 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1876531 PMC1876531]
Online version:[http://dx.doi.org/10.1073/pnas.0702394104 10.1073/pnas.0702394104]
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|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=17483465 PubMed]
!align=left  |Keywords
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Adenosine/metabolism; Adenosine Deaminase/physiology; Amino Acid Sequence; Base Sequence; Cell Line; Cytidine/metabolism; Cytidine Deaminase/physiology; Deamination; Inosine/metabolism; Molecular Sequence Data; RNA Editing; RNA Interference; Uridine/metabolism
 
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==Significance {{HelpIcon|Help:Literature}} ==
==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}
{{LitSignificance}}

Revision as of 18:57, 1 September 2013

Citation

No author listed (2007) An adenosine-to-inosine tRNA-editing enzyme that can perform C-to-U deamination of DNA. Proc. Natl. Acad. Sci. U.S.A. 104:7821-6

Abstract

Adenosine-to-inosine editing in the anticodon of tRNAs is essential for viability. Enzymes mediating tRNA adenosine deamination in bacteria and yeast contain cytidine deaminase-conserved motifs, suggesting an evolutionary link between the two reactions. In trypanosomatids, tRNAs undergo both cytidine-to-uridine and adenosine-to-inosine editing, but the relationship between the two reactions is unclear. Here we show that down-regulation of the Trypanosoma brucei tRNA-editing enzyme by RNAi leads to a reduction in both C-to-U and A-to-I editing of tRNA in vivo. Surprisingly, in vitro, this enzyme can mediate A-to-I editing of tRNA and C-to-U deamination of ssDNA but not both in either substrate. The ability to use both DNA and RNA provides a model for a multispecificity editing enzyme. Notably, the ability of a single enzyme to perform two different deamination reactions also suggests that this enzyme still maintains specificities that would have been found in the ancestor deaminase, providing a first line of evidence for the evolution of editing deaminases.

Links

PubMed PMC1876531 Online version:10.1073/pnas.0702394104

Keywords

Adenosine/metabolism; Adenosine Deaminase/physiology; Amino Acid Sequence; Base Sequence; Cell Line; Cytidine/metabolism; Cytidine Deaminase/physiology; Deamination; Inosine/metabolism; Molecular Sequence Data; RNA Editing; RNA Interference; Uridine/metabolism

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