PMID:16862137: Difference between revisions

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<span style = 'font-size: large'> '''Zaslaver, A, Bren, A, Ronen, M, Itzkovitz, S, Kikoin, I, Shavit, S, Liebermeister, W, Surette, MG and Alon, U'''  (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli.''Nat. Methods'' '''3''':623-8</span>
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|{{TableHeadingColor}} |'''Abstract''' || <protect>E. coli is widely used for systems biology research; there exists a need, however, for tools that can be used to accurately and comprehensively measure expression dynamics in individual living cells. To address this we present a library of transcriptional fusions of gfp to each of about 2,000 different promoters in E. coli K12, covering the great majority of the promoters in the organism. Each promoter fusion is expressed from a low-copy plasmid. We demonstrate that this library can be used to obtain highly accurate dynamic measurements of promoter activity on a genomic scale, in a glucose-lactose diauxic shift experiment. The library allowed detection of about 80 previously uncharacterized transcription units in E. coli, including putative internal promoters within previously known operons, such as the lac operon. This library can serve as a tool for accurate, high-resolution analysis of transcription networks in living E. coli cells.</protect>
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'''No author listed'''  (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. ''Nat. Methods'' '''3''':623-8
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!align=left  |Abstract
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E. coli is widely used for systems biology research; there exists a need, however, for tools that can be used to accurately and comprehensively measure expression dynamics in individual living cells. To address this we present a library of transcriptional fusions of gfp to each of about 2,000 different promoters in E. coli K12, covering the great majority of the promoters in the organism. Each promoter fusion is expressed from a low-copy plasmid. We demonstrate that this library can be used to obtain highly accurate dynamic measurements of promoter activity on a genomic scale, in a glucose-lactose diauxic shift experiment. The library allowed detection of about 80 previously uncharacterized transcription units in E. coli, including putative internal promoters within previously known operons, such as the lac operon. This library can serve as a tool for accurate, high-resolution analysis of transcription networks in living E. coli cells.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16862137 PubMed]
Online version:[http://dx.doi.org/10.1038/nmeth895 10.1038/nmeth895]
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|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16862137 PubMed]<br/>Online version:[http://dx.doi.org/10.1038/nmeth895 10.1038/nmeth895]
!align=left  |Keywords
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Escherichia coli/metabolism; Escherichia coli Proteins/analysis; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Profiling/methods; Peptide Library; Spectrometry, Fluorescence/methods; Transcription Factors/analysis; Transcription Factors/genetics; Transcription Factors/metabolism
 
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==Significance {{HelpIcon|Help:Literature}} ==
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Revision as of 18:53, 1 September 2013

Citation

No author listed (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3:623-8

Abstract

E. coli is widely used for systems biology research; there exists a need, however, for tools that can be used to accurately and comprehensively measure expression dynamics in individual living cells. To address this we present a library of transcriptional fusions of gfp to each of about 2,000 different promoters in E. coli K12, covering the great majority of the promoters in the organism. Each promoter fusion is expressed from a low-copy plasmid. We demonstrate that this library can be used to obtain highly accurate dynamic measurements of promoter activity on a genomic scale, in a glucose-lactose diauxic shift experiment. The library allowed detection of about 80 previously uncharacterized transcription units in E. coli, including putative internal promoters within previously known operons, such as the lac operon. This library can serve as a tool for accurate, high-resolution analysis of transcription networks in living E. coli cells.

Links

PubMed Online version:10.1038/nmeth895

Keywords

Escherichia coli/metabolism; Escherichia coli Proteins/analysis; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Profiling/methods; Peptide Library; Spectrometry, Fluorescence/methods; Transcription Factors/analysis; Transcription Factors/genetics; Transcription Factors/metabolism

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Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status
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