PMID:16858726: Difference between revisions
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| | !align=left |Citation | ||
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'''No author listed''' (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. ''Electrophoresis'' '''27''':3306-21 | |||
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!align=left |Abstract | |||
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Study of the complexome - all the protein complexes of the cell - is essential for a better understanding and more global vision of cell function. Using two-dimensional blue native/SDS-PAGE (2-D BN/SDS-PAGE) technology, the cytosolic and membrane protein complexes of Escherichia coli were separated. Then, the different partners of each protein complex were identified by LC-MS/MS. In this report, 306 protein complexes were separated and identified. Among these protein complexes, 50 heteromultimeric and 256 homomultimeric protein complexes were found. Among the 50 heteromultimeric protein complexes, 18 previously described protein complexes validate the technology. In this study, 109 new protein complexes were found, providing insight into the function of previously uncharacterized bacterial proteins. | |||
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!align=left |Links | |||
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16858726 PubMed] | |||
Online version:[http://dx.doi.org/10.1002/elps.200500912 10.1002/elps.200500912] | |||
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|{{ | !align=left |Keywords | ||
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Chromatography, Liquid; Electrophoresis, Gel, Two-Dimensional/methods; Electrophoresis, Polyacrylamide Gel/methods; Escherichia coli/chemistry; Escherichia coli/metabolism; Escherichia coli Proteins/analysis; Escherichia coli Proteins/classification; Escherichia coli Proteins/metabolism; Mass Spectrometry; Proteome/analysis; Proteome/metabolism | |||
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==Significance {{HelpIcon|Help:Literature}} == | ==Significance {{HelpIcon|Help:Literature}} == | ||
{{LitSignificance}} | {{LitSignificance}} | ||
Revision as of 18:53, 1 September 2013
| Citation |
No author listed (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27:3306-21 |
|---|---|
| Abstract |
Study of the complexome - all the protein complexes of the cell - is essential for a better understanding and more global vision of cell function. Using two-dimensional blue native/SDS-PAGE (2-D BN/SDS-PAGE) technology, the cytosolic and membrane protein complexes of Escherichia coli were separated. Then, the different partners of each protein complex were identified by LC-MS/MS. In this report, 306 protein complexes were separated and identified. Among these protein complexes, 50 heteromultimeric and 256 homomultimeric protein complexes were found. Among the 50 heteromultimeric protein complexes, 18 previously described protein complexes validate the technology. In this study, 109 new protein complexes were found, providing insight into the function of previously uncharacterized bacterial proteins. |
| Links |
PubMed Online version:10.1002/elps.200500912 |
| Keywords |
Chromatography, Liquid; Electrophoresis, Gel, Two-Dimensional/methods; Electrophoresis, Polyacrylamide Gel/methods; Escherichia coli/chemistry; Escherichia coli/metabolism; Escherichia coli Proteins/analysis; Escherichia coli Proteins/classification; Escherichia coli Proteins/metabolism; Mass Spectrometry; Proteome/analysis; Proteome/metabolism |
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Significance
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Useful Materials and Methods
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Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
TpiA |
GO:0005737 |
cytoplasm |
IDA: Inferred from Direct Assay |
C |
Supplemental information, row 163 of table. |
complete | ||
| GO:0005624 |
membrane fraction |
IDA: Inferred from Direct Assay |
C |
complete | ||||
|
YbbN |
GO:0005737 |
cytoplasm |
IDA: Inferred from Direct Assay |
C |
Supplemental information. |
complete | ||
|
tdcE, 2-ketobutyrate formate-lyase/pyruvate formate-lyase 4, inactive |
GO:0005624 |
membrane fraction |
IDA: Inferred from Direct Assay |
C |
Table 3. Unknown cytosolic and membrane homomultimeric complexes, tdcE is complex 299. |
complete | ||
|
AceF, the "E2" or "core" component of the pyruvate dehydrogenase multienzyme complex |
GO:0005737 |
cytoplasm |
IDA: Inferred from Direct Assay |
C |
Table 1. Known cytosolic (C) and membrane (M) heteromultimeric protein complexes, complex 3. |
complete | ||
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References
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