PMID:16392005: Difference between revisions

Revision as of 18:51, 1 September 2013

Citation	No author listed (2006) Gene expression profiling of intrinsic thermotolerance in Escherichia coli. Curr. Microbiol. 52:50-4
Abstract	DNA microarrays were employed to compare gene expression in a thermotolerant, nalidixic acid-resistant mutant of Escherichia coli with that of the parental strain. When grown at 37 degrees C, up-regulated genes in the mutant included those coding for multiple antibiotic resistance proteins and enzymes for the degradation of small molecules, whereas among the down-regulated genes were those coding for fimbrial, flagellar, and outer membrane proteins as well as sigma 38. When the mutant grown at 42 degrees C was compared to the mutant grown at 37 degrees C, enhanced expression of several genes coding for flagellar proteins was detected. Reverse transcriptase-polymerase chain reaction analysis of selected genes confirmed results obtained with microarrays.
Links	PubMed Online version:10.1007/s00284-005-4578-6
Keywords	Adaptation, Physiological; Anti-Bacterial Agents/pharmacology; Drug Resistance, Bacterial; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins/genetics; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Hot Temperature; Mutation; Nalidixic Acid/pharmacology; Oligonucleotide Array Sequence Analysis; RNA, Bacterial/analysis; RNA, Messenger/analysis; Reverse Transcriptase Polymerase Chain Reaction
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