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| | !align=left |Citation | ||
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'''No author listed''' (2006) Gene expression profiling of intrinsic thermotolerance in Escherichia coli. ''Curr. Microbiol.'' '''52''':50-4 | |||
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!align=left |Abstract | |||
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DNA microarrays were employed to compare gene expression in a thermotolerant, nalidixic acid-resistant mutant of Escherichia coli with that of the parental strain. When grown at 37 degrees C, up-regulated genes in the mutant included those coding for multiple antibiotic resistance proteins and enzymes for the degradation of small molecules, whereas among the down-regulated genes were those coding for fimbrial, flagellar, and outer membrane proteins as well as sigma 38. When the mutant grown at 42 degrees C was compared to the mutant grown at 37 degrees C, enhanced expression of several genes coding for flagellar proteins was detected. Reverse transcriptase-polymerase chain reaction analysis of selected genes confirmed results obtained with microarrays. | |||
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!align=left |Links | |||
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16392005 PubMed] | |||
Online version:[http://dx.doi.org/10.1007/s00284-005-4578-6 10.1007/s00284-005-4578-6] | |||
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|{{ | !align=left |Keywords | ||
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Adaptation, Physiological; Anti-Bacterial Agents/pharmacology; Drug Resistance, Bacterial; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins/genetics; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Hot Temperature; Mutation; Nalidixic Acid/pharmacology; Oligonucleotide Array Sequence Analysis; RNA, Bacterial/analysis; RNA, Messenger/analysis; Reverse Transcriptase Polymerase Chain Reaction | |||
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==Significance {{HelpIcon|Help:Literature}} == | ==Significance {{HelpIcon|Help:Literature}} == | ||
{{LitSignificance}} | {{LitSignificance}} | ||
Revision as of 18:51, 1 September 2013
| Citation |
No author listed (2006) Gene expression profiling of intrinsic thermotolerance in Escherichia coli. Curr. Microbiol. 52:50-4 |
|---|---|
| Abstract |
DNA microarrays were employed to compare gene expression in a thermotolerant, nalidixic acid-resistant mutant of Escherichia coli with that of the parental strain. When grown at 37 degrees C, up-regulated genes in the mutant included those coding for multiple antibiotic resistance proteins and enzymes for the degradation of small molecules, whereas among the down-regulated genes were those coding for fimbrial, flagellar, and outer membrane proteins as well as sigma 38. When the mutant grown at 42 degrees C was compared to the mutant grown at 37 degrees C, enhanced expression of several genes coding for flagellar proteins was detected. Reverse transcriptase-polymerase chain reaction analysis of selected genes confirmed results obtained with microarrays. |
| Links |
PubMed Online version:10.1007/s00284-005-4578-6 |
| Keywords |
Adaptation, Physiological; Anti-Bacterial Agents/pharmacology; Drug Resistance, Bacterial; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins/genetics; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Hot Temperature; Mutation; Nalidixic Acid/pharmacology; Oligonucleotide Array Sequence Analysis; RNA, Bacterial/analysis; RNA, Messenger/analysis; Reverse Transcriptase Polymerase Chain Reaction |
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| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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