PMID:1055366: Difference between revisions

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!align=left  |Citation
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'''Nash, HA '''  (1975) Integrative recombination of bacteriophage lambda DNA in vitro. ''Proc. Natl. Acad. Sci. U.S.A.'' '''72''':1072-6
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!align=left  |Abstract
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An in vitro system for the production of integrative recombinant DNA of bacteriophage lambda is described. The in vitro recombination mimics the in vivo integration of viral DNA into host DNA in its requirement for int gene product, for the presence of a thermolabile component, and for the limitation of the recombination to a pair of specialized sites (attachment sites) on the DNA. The enzymes are extracted from Escherichia coli containing phage lambda gene products. The substrate is the DNA from lambda-attB-attP, a phage variant that contains two attachment sites on the same chromosome. The product is a recombinant phage chromosome that has lost the DNA between the attachment sites. The parental and recombinant DNA are distinguished following transfection to mature phage in spheroplasts. The reaction requires ATP, Mg++, spermidine, and a monovalent cation. Recombination occurs preferentially between attachment sites on the same molecule. The enzymatic activity is completely inhibited by extracts containing xis gene product.
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!align=left  |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1055366 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC432468 PMC432468]
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!align=left  |Keywords
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Adenosine Triphosphate/metabolism; Chromosome Mapping; Coliphages; DNA, Viral/metabolism; Kinetics; Magnesium/metabolism; Potassium/metabolism; Recombination, Genetic; Spermidine/metabolism
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==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}
== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}
==Annotations {{HelpIcon|Help:Literature}}==
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!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}
==See also==
==References==
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<references/>

Revision as of 09:58, 21 February 2013

Citation

Nash, HA (1975) Integrative recombination of bacteriophage lambda DNA in vitro. Proc. Natl. Acad. Sci. U.S.A. 72:1072-6

Abstract

An in vitro system for the production of integrative recombinant DNA of bacteriophage lambda is described. The in vitro recombination mimics the in vivo integration of viral DNA into host DNA in its requirement for int gene product, for the presence of a thermolabile component, and for the limitation of the recombination to a pair of specialized sites (attachment sites) on the DNA. The enzymes are extracted from Escherichia coli containing phage lambda gene products. The substrate is the DNA from lambda-attB-attP, a phage variant that contains two attachment sites on the same chromosome. The product is a recombinant phage chromosome that has lost the DNA between the attachment sites. The parental and recombinant DNA are distinguished following transfection to mature phage in spheroplasts. The reaction requires ATP, Mg++, spermidine, and a monovalent cation. Recombination occurs preferentially between attachment sites on the same molecule. The enzymatic activity is completely inhibited by extracts containing xis gene product.

Links

PubMed PMC432468

Keywords

Adenosine Triphosphate/metabolism; Chromosome Mapping; Coliphages; DNA, Viral/metabolism; Kinetics; Magnesium/metabolism; Potassium/metabolism; Recombination, Genetic; Spermidine/metabolism

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Significance

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Useful Materials and Methods

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Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status
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EcoliWiki Links

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See also

References

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