PMID:3003084: Difference between revisions

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!align=left  |Citation
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'''Ohki, M, Tamura, F, Nishimura, S and Uchida, H'''  (1986) Nucleotide sequence of the Escherichia coli dnaJ gene and purification of the gene product.''J. Biol. Chem.'' '''261''':1778-81
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!align=left  |Abstract
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The dnaJ and dnaK genes are essential for replication of Escherichia coli DNA, and they constitute an operon, dnaJ being downstream from dnaK. The amount of the dnaJ protein in E. coli is substantially less than that of the dnaK protein, which is produced abundantly. In order to construct a system that over-produces the dnaJ protein, we started our study by determining the DNA sequence of the entire dnaJ gene, and an operon fusion was constructed by inserting the gene downstream of the lambda PL promoter of an expression vector plasmid, pPL-lambda. Cells containing the recombinant plasmid produced dnaJ protein amounting to 2% of the total cellular protein when cells were induced. The overproduced protein was purified, and Edman degradation of the protein indicated that the NH2-terminal methionine was found to be processed. From the DNA sequence of the dnaJ gene, the processed gene product is composed of 375 amino acid residues, and its molecular weight is calculated to be 40,975.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3003084 PubMed]
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!align=left  |Keywords
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Amino Acid Sequence; Bacterial Proteins; Base Sequence; DNA Replication; Escherichia coli; Escherichia coli Proteins; Genes, Bacterial; HSP40 Heat-Shock Proteins; Heat-Shock Proteins; Methionine; Molecular Weight; Recombinant Proteins; Sequence Homology, Nucleic Acid
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==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}
== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}
==Annotations {{HelpIcon|Help:Literature}}==
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!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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Revision as of 16:37, 21 May 2012

Citation

Ohki, M, Tamura, F, Nishimura, S and Uchida, H (1986) Nucleotide sequence of the Escherichia coli dnaJ gene and purification of the gene product.J. Biol. Chem. 261:1778-81

Abstract

The dnaJ and dnaK genes are essential for replication of Escherichia coli DNA, and they constitute an operon, dnaJ being downstream from dnaK. The amount of the dnaJ protein in E. coli is substantially less than that of the dnaK protein, which is produced abundantly. In order to construct a system that over-produces the dnaJ protein, we started our study by determining the DNA sequence of the entire dnaJ gene, and an operon fusion was constructed by inserting the gene downstream of the lambda PL promoter of an expression vector plasmid, pPL-lambda. Cells containing the recombinant plasmid produced dnaJ protein amounting to 2% of the total cellular protein when cells were induced. The overproduced protein was purified, and Edman degradation of the protein indicated that the NH2-terminal methionine was found to be processed. From the DNA sequence of the dnaJ gene, the processed gene product is composed of 375 amino acid residues, and its molecular weight is calculated to be 40,975.

Links

PubMed

Keywords

Amino Acid Sequence; Bacterial Proteins; Base Sequence; DNA Replication; Escherichia coli; Escherichia coli Proteins; Genes, Bacterial; HSP40 Heat-Shock Proteins; Heat-Shock Proteins; Methionine; Molecular Weight; Recombinant Proteins; Sequence Homology, Nucleic Acid

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Significance

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Useful Materials and Methods

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Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status
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EcoliWiki Links

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See also

References

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