PMID:21467222: Difference between revisions
Jump to navigation
Jump to search
New PMID: Page! |
Fill PMID: Page! |
||
| Line 1: | Line 1: | ||
{{RightTOC}} | |||
<protect> | |||
<span style = 'font-size: large'> '''Hassaninasab, A, Hashimoto, Y, Tomita-Yokotani, K and Kobayashi, M''' (2011) Discovery of the curcumin metabolic pathway involving a unique enzyme in an intestinal microorganism.''Proc. Natl. Acad. Sci. U.S.A.'' '''108''':6615-20</span> | |||
{|{{Prettytable}} align = 'center' width= '70%' | |||
|- | |||
|{{TableHeadingColor}} |'''Abstract''' || <protect>Polyphenol curcumin, a yellow pigment, derived from the rhizomes of a plant (Curcuma longa Linn) is a natural antioxidant exhibiting a variety of pharmacological activities and therapeutic properties. It has long been used as a traditional medicine and as a preservative and coloring agent in foods. Here, curcumin-converting microorganisms were isolated from human feces, the one exhibiting the highest activity being identified as Escherichia coli. We are thus unique in discovering that E. coli was able to act on curcumin. The curcumin-converting enzyme was purified from E. coli and characterized. The native enzyme had a molecular mass of about 82 kDa and consisted of two identical subunits. The enzyme has a narrow substrate spectrum, preferentially acting on curcumin. The microbial metabolism of curcumin by the purified enzyme was found to comprise a two-step reduction, curcumin being converted NADPH-dependently into an intermediate product, dihydrocurcumin, and then the end product, tetrahydrocurcumin. We named this enzyme "NADPH-dependent curcumin/dihydrocurcumin reductase" (CurA). The gene (curA) encoding this enzyme was also identified. A homology search with the BLAST program revealed that a unique enzyme involved in curcumin metabolism belongs to the medium-chain dehydrogenase/reductase superfamily.</protect> | |||
|- | |||
|{{TableHeadingColor}} nowrap |'''Links''' || | |||
* [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=21467222 PubMed] | |||
Online version:[http://dx.doi.org/10.1073/pnas.1016217108 10.1073/pnas.1016217108] | |||
|} | |||
</protect> | |||
==Significance {{HelpIcon|Help:Literature}} == | |||
{{LitSignificance}} | |||
== Useful Materials and Methods {{HelpIcon|Help:Literature}}== | |||
{{LitMaterials}} | |||
==Annotations {{HelpIcon|Help:Literature}}== | |||
<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.179506.X4e41a8e6bceed--> | |||
<!-- | |||
****************************************************************************************** | |||
* | |||
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | |||
* | |||
****************************************************************************************** --> | |||
{| id="X4e41a8e6bceed" class=" tableEdit GO_table_reference dataTable" | |||
|- {{table heading style}} | |||
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status | |||
|- class="tableEdit_footer" | |||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.179506.X4e41a8e6bceed&page=179506&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table]</span> || || || || || || || || | |||
|} | |||
<!--box uid=63ce1649aab8b3180235b0af7618f667.179506.X4e41a8e6bceed--></protect> | |||
==EcoliWiki Links {{HelpIcon|Help:Literature}} == | |||
{{LitLinks}} | |||
==See also== | |||
==References== | |||
{{RefHelp}} | |||
<references/> | |||
Revision as of 21:38, 9 August 2011
Hassaninasab, A, Hashimoto, Y, Tomita-Yokotani, K and Kobayashi, M (2011) Discovery of the curcumin metabolic pathway involving a unique enzyme in an intestinal microorganism.Proc. Natl. Acad. Sci. U.S.A. 108:6615-20
| Abstract | <protect>Polyphenol curcumin, a yellow pigment, derived from the rhizomes of a plant (Curcuma longa Linn) is a natural antioxidant exhibiting a variety of pharmacological activities and therapeutic properties. It has long been used as a traditional medicine and as a preservative and coloring agent in foods. Here, curcumin-converting microorganisms were isolated from human feces, the one exhibiting the highest activity being identified as Escherichia coli. We are thus unique in discovering that E. coli was able to act on curcumin. The curcumin-converting enzyme was purified from E. coli and characterized. The native enzyme had a molecular mass of about 82 kDa and consisted of two identical subunits. The enzyme has a narrow substrate spectrum, preferentially acting on curcumin. The microbial metabolism of curcumin by the purified enzyme was found to comprise a two-step reduction, curcumin being converted NADPH-dependently into an intermediate product, dihydrocurcumin, and then the end product, tetrahydrocurcumin. We named this enzyme "NADPH-dependent curcumin/dihydrocurcumin reductase" (CurA). The gene (curA) encoding this enzyme was also identified. A homology search with the BLAST program revealed that a unique enzyme involved in curcumin metabolism belongs to the medium-chain dehydrogenase/reductase superfamily. |
|- |bgcolor='#CCCCFF' nowrap |Links ||
Online version:10.1073/pnas.1016217108 |} </protect>
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.