PMID:15916808: Difference between revisions
Jump to navigation
Jump to search
Update TableEdit tables. |
/Volumes/r3/t3/ul/working/touchTables/touch_all_tables.php |
||
| Line 26: | Line 26: | ||
****************************************************************************************** --> | ****************************************************************************************** --> | ||
{| id="U47a937132450e" class=" tableEdit GO_table_reference" | {| id="U47a937132450e" class=" tableEdit GO_table_reference" | ||
|- | |- {{table heading style}} | ||
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status | !|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status | ||
Revision as of 20:27, 17 June 2010
Mu, W, Zhang, D, Xu, L, Luo, Z and Wang, Y (2005) Activity assay of His-tagged E. coli DNA photolyase by RP-HPLC and SE-HPLC.J. Biochem. Biophys. Methods 63:111-24
| Abstract | Escherichia coli DNA photolyase was expressed as C-terminal 6x histidine-fused protein. Purification of His-tagged E. coli DNA photolyase was developed using immobilized metal affinity chromatography with Chelating Sepharose Fast Flow. By one-step affinity chromatography, approximate 4.6 mg DNA photolyase was obtained from 400 ml E. coli culture. The purified His-tagged enzyme was combined with two chromophors, FADH and MTHF. Using the oligonucleotide containing cyclobutane pyrimidine dimer as substrate, both reversed-phase high-performance liquid chromatography and size-exclusion high-performance liquid chromatography were developed to measure the enzyme activity. The enzyme was found to be able to repair the cyclobutane pyrimidine dimer with the turnover rate of 2.4 dimers/photolyase molecule/min. |
| Links | PubMed |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.