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<span style = 'font-size: large'> '''Bischof, O, Kruft, V and Wittmann-Liebold, B''' (1994) Analysis of the puromycin binding site in the 70 S ribosome of Escherichia coli at the peptide level.''J. Biol. Chem.'' '''269''':18315-9</span> | |||
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|{{TableHeadingColor}} |'''Abstract''' || <protect>Photoinduced cross-linking of Escherichia coli 70 S ribosomes with [3H]puromycin has led to the labeling of ribosomal proteins S7, S14, S18, L18, and L29. Proteolytic fragmentation of these proteins and separation of the peptide mixtures by C18 reversed-phase high performance liquid chromatography resulted in six puromycin-labeled peptides which were applied to sequence analysis. The following peptides were found labeled: Pro1-Lys10 of S7, Ala28-Lys46 and Ala7-Lys11 of S14, Asp24-Lys29 of S18, Tyr64-Lys68 of L18, and Thr55-Lys60 of L29. For the first time the molecular environment of an antibiotic in the procaryotic ribosome is presented at the peptide level.</protect> | |||
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8034577 PubMed]<br/> | |||
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Revision as of 17:28, 1 February 2010
Bischof, O, Kruft, V and Wittmann-Liebold, B (1994) Analysis of the puromycin binding site in the 70 S ribosome of Escherichia coli at the peptide level.J. Biol. Chem. 269:18315-9
| Abstract | <protect>Photoinduced cross-linking of Escherichia coli 70 S ribosomes with [3H]puromycin has led to the labeling of ribosomal proteins S7, S14, S18, L18, and L29. Proteolytic fragmentation of these proteins and separation of the peptide mixtures by C18 reversed-phase high performance liquid chromatography resulted in six puromycin-labeled peptides which were applied to sequence analysis. The following peptides were found labeled: Pro1-Lys10 of S7, Ala28-Lys46 and Ala7-Lys11 of S14, Asp24-Lys29 of S18, Tyr64-Lys68 of L18, and Thr55-Lys60 of L29. For the first time the molecular environment of an antibiotic in the procaryotic ribosome is presented at the peptide level. |
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|bgcolor='#CCCCFF' nowrap |Links || PubMed
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