Phage T7: Difference between revisions
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Gene 1: specifies RNA polymerase; Genes 2-6: affect kinetics of DNA synthesis; Genes 7-17: specify proteins found within partially and fully assembled particles; Genes 18-19: involved in the maturation of the DNA | Gene 1: specifies RNA polymerase; Genes 2-6: affect kinetics of DNA synthesis; Genes 7-17: specify proteins found within partially and fully assembled particles; Genes 18-19: involved in the maturation of the DNA | ||
General specifications of what the genes coded for were found using Amber Mutations. | |||
== Life Cycle == | == Life Cycle == | ||
Revision as of 02:24, 24 February 2009
Template:Stub Bacteriophage T7 is a virulent phage with an icosahedral head, a short noncontractile tail, and a dsDNA genome of 39937 bp.
Phage structure
Genome structure
39937 bases in a single, linear molecule of dsDNA
Gene 1: specifies RNA polymerase; Genes 2-6: affect kinetics of DNA synthesis; Genes 7-17: specify proteins found within partially and fully assembled particles; Genes 18-19: involved in the maturation of the DNA
General specifications of what the genes coded for were found using Amber Mutations.
Life Cycle
Host recognition
Lipopolysaccharides on the external cell wall of the host cell provide recognition for the Phage
DNA entry
A protrusion of the Phage (the tail) lengthens and attaches the Phage to the Host cell, and proceeds to send the virus' dsDNA through the channel. After the first 850 to 1000 base pairs, the insertion is paused until an injection promoter allows it to continue.
Early gene expression
DNA replication
T7 breaks down the host cell's DNA to its nucleotides for it's own use (using an endonuclease and exonuclease coded by genes 3 and 6, respectively).
Late transcription
Packaging
Lysis
See also
- Links to T7 gene pages in EcoliWiki
- Studier [1] wrote an overview of t7 biology in genetics in 1972.
- Krüger DH and Schroeder C (1981)[2]
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Studier, FW (1972) Bacteriophage T7. Science 176 367-76 PubMed
- ↑ Krüger, DH & Schroeder, C (1981) Bacteriophage T3 and bacteriophage T7 virus-host cell interactions. Microbiol. Rev. 45 9-51 PubMed
- ↑ Serwer, P et al. (2008) Evidence for bacteriophage T7 tail extension during DNA injection. BMC Res Notes 1 36 PubMed