yiaF:Quickview: Difference between revisions

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===Notes===
===Notes===
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YiaF has been identified as a membrane-associated protein by 2D-MS, with a non-uniform membrane distribution, indicating both polar and septal bias. Interpretation of YiaF results in Lai et al. (2004) was complicated by use of a predicted start site at Met(-40). A null mutant has no obvious growth defect (Lai, 2004). Reported MS detection of a fragment from 19R-30E (stated as 50R-69E in Lai, 2004) may indicate the presence of an uncleaved precursor form in minicell membrane preparations. First 25 aa are predicted to be a type II signal peptide. HT_Cmplx47_Mem: MscS+YiaF.<ref name='LIB:EcoGene'/>





Revision as of 02:02, 3 March 2009

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
References Suggestions
Gene Name

yiaF (synonyms: ECK3541, b3554, JW5655)[1]

Product(s)

YiaF product[1]: conserved protein product, B3554 product

Description

conserved protein[2][3]

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): yiaF[2]

Regulation/Activity


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Notes

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YiaF has been identified as a membrane-associated protein by 2D-MS, with a non-uniform membrane distribution, indicating both polar and septal bias. Interpretation of YiaF results in Lai et al. (2004) was complicated by use of a predicted start site at Met(-40). A null mutant has no obvious growth defect (Lai, 2004). Reported MS detection of a fragment from 19R-30E (stated as 50R-69E in Lai, 2004) may indicate the presence of an uncleaved precursor form in minicell membrane preparations. First 25 aa are predicted to be a type II signal peptide. HT_Cmplx47_Mem: MscS+YiaF.[4]



References

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  1. ↑ 1.0 1.1 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.