yhdJ:Quickview: Difference between revisions
imported>Wikientrybot Move help links into the <above> tag inside templates. |
imported>Wikientrybot Adding EcoGene Comments |
||
| Line 60: | Line 60: | ||
===Notes=== | ===Notes=== | ||
{{BacktoTop}} | {{BacktoTop}} | ||
In vitro DNA adenine methyltransferase activity has been demonstrated for YhdJ; overexpression of YhdJ leads to protection of chromosomal DNA from NsiI digestion, but DNA from wild type cells is not protected (Broadbent (2007). DNA from a DNA methyltransferase deficient strain (dam-, dcm-, EcoK-) does not react with anti-N6-methylA or anti-N4-methylC antibodies when YhdJ is expressed from the pBR322 Tet promoter; the yhdJ-containing pBR322 derivative was not resistant to a variety of restriction (personal communication: R. Vaisvila, S. Stickel and R.J. Roberts, unpublished results, April, 2006). The Kossykh (2004) report describing yhdJ as ccrM, an adenine methyltransferase, has been retracted.<ref name='LIB:EcoGene'/> | |||
Revision as of 01:15, 3 March 2009
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| References | Suggestions |
| Gene Name | |
|---|---|
| Product(s) |
YhdJ product[1]: predicted methyltransferase product, B3262 product, CcrM product |
| Description |
predicted methyltransferase[2] DNA adenine methyltransferase[3] |
| Knock-Out Phenotype | |
| Regulation/Expression | |
| Regulation/Activity |
|
| edit table |
See Help:Quickview for help with entering information in the Quickview table.
Notes
In vitro DNA adenine methyltransferase activity has been demonstrated for YhdJ; overexpression of YhdJ leads to protection of chromosomal DNA from NsiI digestion, but DNA from wild type cells is not protected (Broadbent (2007). DNA from a DNA methyltransferase deficient strain (dam-, dcm-, EcoK-) does not react with anti-N6-methylA or anti-N4-methylC antibodies when YhdJ is expressed from the pBR322 Tet promoter; the yhdJ-containing pBR322 derivative was not resistant to a variety of restriction (personal communication: R. Vaisvila, S. Stickel and R.J. Roberts, unpublished results, April, 2006). The Kossykh (2004) report describing yhdJ as ccrM, an adenine methyltransferase, has been retracted.[4]
References
- ↑ 1.0 1.1 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.