DH10B: Difference between revisions
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== Strain == | == Strain == | ||
DH10B | DH10B | ||
== Genotype == | == Genotype == | ||
F<sup>-</sup> ''mcr''A Δ(''mrr''-''hsd''RMS-''mcr''BC) Φ80d''lac''ZΔM15 Δ''lac''X74 ''end''A1 ''rec''A1 ''deo''R Δ(''ara'',''leu'')7697 ''ara''D139 ''gal''U ''gal''K ''nup''G ''rps''L λ<sup>-</sup><br /> | |||
F<sup>-</sup> ''mcr''A Δ(''mrr''-''hsd''RMS-''mcr''BC) Φ80d''lac''ZΔM15 Δ''lac''X74 ''end''A1 ''rec''A1 ''deo''R Δ(''ara'',''leu'')7697 ''ara''D139 ''gal''U ''gal''K ''nup''G ''rps''L λ<sup>-</sup> | From J Jessee.<ref name='PMID:2162051'/> | ||
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== Comments == | == Comments == | ||
| Line 18: | Line 14: | ||
== References == | == References == | ||
1. S G Grant, J Jessee, F R Bloom, and D Hanahan. Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc Natl Acad Sci. 1990. 87(12):4645–4649.< | 1. S G Grant, J Jessee, F R Bloom, and D Hanahan. Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc Natl Acad Sci. 1990. 87(12):4645–4649. | ||
<references/> | |||
Revision as of 21:18, 17 September 2008
Strain
DH10B
Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara,leu)7697 araD139 galU galK nupG rpsL λ-
From J Jessee.[1]
Comments
host for pUC and other α-complementation vectors; pBR322
useful for generating genomic libraries containing methylated cytosine or adenine residues
useful for plasmid rescue procedures
Copyed from GIBCO
References
1. S G Grant, J Jessee, F R Bloom, and D Hanahan. Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc Natl Acad Sci. 1990. 87(12):4645–4649.