DH10B: Difference between revisions
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== Strain == | == Strain == | ||
DH10B | DH10B | ||
== Genotype == | == Genotype == | ||
| Line 8: | Line 6: | ||
F<sup>-</sup> ''mcr''A Δ(''mrr''-''hsd''RMS-''mcr''BC) Φ80d''lac''ZΔM15 Δ''lac''X74 ''end''A1 ''rec''A1 ''deo''R Δ(''ara'',''leu'')7697 ''ara''D139 ''gal''U ''gal''K ''nup''G ''rps''L λ<sup>-</sup> | F<sup>-</sup> ''mcr''A Δ(''mrr''-''hsd''RMS-''mcr''BC) Φ80d''lac''ZΔM15 Δ''lac''X74 ''end''A1 ''rec''A1 ''deo''R Δ(''ara'',''leu'')7697 ''ara''D139 ''gal''U ''gal''K ''nup''G ''rps''L λ<sup>-</sup> | ||
From GIBCO<sup>TM</sup> website | |||
== Comments == | == Comments == | ||
| Line 19: | Line 15: | ||
Copyed from GIBCO | Copyed from GIBCO | ||
== References == | == References == | ||
1. Lorow, D. & Jessee, J. (1990) ''Focus''. 12,19 | 1. Lorow, D. & Jessee, J. (1990) ''Focus''. 12,19 | ||
Revision as of 16:18, 17 September 2008
Strain
DH10B
Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara,leu)7697 araD139 galU galK nupG rpsL λ-
From GIBCOTM website
Comments
host for pUC and other α-complementation vectors; pBR322 useful for generating genomic libraries containing methylated cytosine or adenine residues useful for plasmid rescue procedures
Copyed from GIBCO
References
1. Lorow, D. & Jessee, J. (1990) Focus. 12,19