PMID:6792186

Jagura-Burdzy, G and Hulanicka, D (1981) Use of gene fusions to study expression of cysB, the regulatory gene of the cysteine regulon.J. Bacteriol. 147:744-51

Abstract

Strains of escherichia coli were constructed in which the lacZ gene is fused to cysB, the positive regulator gene of the cysteine regulon. The fusion strains were used to study the regulation of the cysB gene by assaying the fused lacZ gene product. The introduction of a cysB allele, either on a plasmid or on an episome to the fusion strains, resulted in the decrease of beta-galactosidase activity. This implies that the cysB gene expression is autoregulated by its own product. The direction of cysB gene transcription was determined to be clockwise.

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Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
cysB:Gene_Product(s)		GO:0016564	transcription repressor activity	IDA: Inferred from Direct Assay		F		required field missing
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