PMID:2678006: Difference between revisions
Jump to navigation
Jump to search
Edited by wikibot |
imported>Wikientrybot Fix links due to TableEditLinks.php changes. |
||
| Line 21: | Line 21: | ||
<!-- | <!-- | ||
****************************************************************************************** | ****************************************************************************************** | ||
* | * | ||
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | ||
* | * | ||
****************************************************************************************** --> | ****************************************************************************************** --> | ||
{|class = 'sortable' | {|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" | ||
|- {{table heading style}} | |- {{table heading style}} | ||
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status | !|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status | ||
|-class='sortbottom' | |-class='sortbottom' | ||
|[{{SERVER}}{{SCRIPTPATH}} | |[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.G47ab2e211f5c0&page=0&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || || | ||
|} | |} | ||
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.G47ab2e211f5c0--> | <!--box uid=63ce1649aab8b3180235b0af7618f667.0.G47ab2e211f5c0--> | ||
Revision as of 04:56, 20 February 2009
Himeno, H, Hasegawa, T, Ueda, T, Watanabe, K, Miura, K and Shimizu, M (1989) Role of the extra G-C pair at the end of the acceptor stem of tRNA(His) in aminoacylation.Nucleic Acids Res. 17:7855-63
| Abstract | All sequenced histidine tRNAs have one additional nucleotide at the 5' end compared with other tRNA species. To investigate the role of this unique structure in aminoacylation, we constructed in vitro transcripts corresponding to the E. coli histidine tRNA sequence and its variants at the G-1-C73 base pair, by using T7 RNA polymerase transcription system. A transcript having a wild-type sequence with no modified bases was a good substrate for histidyl-tRNA synthetase (HisRS), and aminoacylation activity was affected by introduction of a triphosphate at the 5' terminus. Base replacements at position 73 caused a marked decrease of Vmax, and deletion and substitution of the G-1 had a remarkable effect on the aminoacylation. A mutant having an A-1-U73 pair was also not a good substrate for HisRS. Comparison among G-1-deficient mutants showed that A was preferable rather than C as the base at position 73. These data demonstrate that the set of the G-1-C73 pair at the end of the acceptor stem of histidine tRNA is crucial for the catalytic process of aminoacylation. |
| Links | PubMed |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.