PMID:10694574: Difference between revisions
Jump to navigation
Jump to search
Edited by wikibot |
Edited by wikibot |
||
| Line 1: | Line 1: | ||
{{RightTOC}} | {{RightTOC}} | ||
| Line 20: | Line 19: | ||
==Annotations {{HelpIcon|Help:Literature}}== | ==Annotations {{HelpIcon|Help:Literature}}== | ||
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.O46afade532f72--> | <!--box uid=63ce1649aab8b3180235b0af7618f667.0.O46afade532f72--> | ||
[{{SERVER}} | [{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.O46afade532f72&page=0&pagename={{FULLPAGENAMEE}}&type=0 Add GO annotations] | ||
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.O46afade532f72--> | <!--box uid=63ce1649aab8b3180235b0af7618f667.0.O46afade532f72--> | ||
Revision as of 12:03, 30 April 2008
Herz, S, Wungsintaweekul, J, Schuhr, CA, Hecht, S, Luttgen, H, Sagner, S, Fellermeier, M, Eisenreich, W, Zenk, MH, Bacher, A and Rohdich, F (2000) Biosynthesis of terpenoids: YgbB protein converts 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate to 2C-methyl-D-erythritol 2,4-cyclodiphosphate.Proc. Natl. Acad. Sci. U.S.A. 97:2486-90
| Abstract | In many microorganisms, the putative orthologs of the Escherichia coli ygbB gene are tightly linked or fused to putative orthologs of ygbP, which has been shown earlier to be involved in terpenoid biosynthesis. The ygbB gene of E. coli was expressed in a recombinant E. coli strain and was shown to direct the synthesis of a soluble, 17-kDa polypeptide. The recombinant protein was found to convert 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate into 2C-methyl-D-erythritol 2,4-cyclodiphosphate and CMP. The structure of the reaction product was established by NMR spectroscopy using (13)C-labeled substrate samples. The enzyme-catalyzed reaction requires Mn(2+) or Mg(2+) but no other cofactors. Radioactivity from [2-(14)C]2C-methyl-D-erythritol 2,4-cyclodiphosphate was diverted efficiently to carotenoids by isolated chromoplasts from Capsicum annuum and, thus, was established as an intermediate in the deoxyxylulose phosphate pathway of isoprenoid biosynthesis. YgbB protein also was found to convert 4-diphosphocytidyl-2C-methyl-D-erythritol into 2C-methyl-D-erythritol 3,4-cyclophosphate. This compound does not serve as substrate for the formation of carotenoids by isolated chromoplasts and is assumed to be an in vitro product without metabolic relevance. |
| Links | PubMed |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.