pETcoco-1

At a Glance

Type	Expression
Origin of Replication	oriV, oriS
Copy #
Markers	Chloramphenicol
Link to Sequence	Novagen
Comments	The pETcoco-1 expression vector is designed to allow "on command" amplification of vector copy number from single copy (1–2 plasmid copies per cell) to medium copy (20–50 per cell).
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History

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Sources

Novagen

References

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Sequence

       1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag 
      61 gagatataag catgcatcac catcaccatc acggtgctag caaagaaacc gctgctgcga 
     121 aatttgaacg ccagcacatg gactcgccac cgccaactgg tgatgacgac gacaagagcc 
     181 cgggcgatcg catcgatgac gtccaggcgc gccacgtgcg tacggtttcg aagcttgcgg 
     241 ccgcaagcca gccagaactc gctccggaag atcccgagga tctttaatta acctcaggat 
     301 ttaaataacc taggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa 
     361 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc 
     421 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg 
     481 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 
     541 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 
     601 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 
     661 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 
     721 tcacggccgc cgcaatgctt gcataatgtg gcctgtcaaa tggacgaagc agggattctg 
     781 caaaccctat gctactccgt caagccgtca attgtctgat tcgttaccaa ttatgacaac 
     841 ttgacggcta catcattcac tttttcttca caaccggcac gaaactcgct cgggctggcc 
     901 ccggtgcatt ttttaaatac tcgcgagaaa tagagttgat cgtcaaaacc aacattgcga 
     961 ccgacggtgg cgataggcat ccgggtagtg ctcaaaagca gcttcgcctg gctgatacgt 
    1021 tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga 
    1081 cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc 
    1141 aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg cggatggagc 
    1201 gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc 
    1261 agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg 
    1321 aaatgcggct ggtgcgcttc atccgggcga aagaaacccg tattggcaaa tattgacggc 
    1381 cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat 
    1441 tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata 
    1501 tcacccggtc ggcagacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg 
    1561 gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa 
    1621 ccgttggcct caatcggcgt taaacccgcc accagatggg cgttaaacga gtatcccggc 
    1681 agcaggggat cattttgcgc ttcagccata cttttcatac tcccaccatt cagagaagaa 
    1741 accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg gctcttctcg 
    1801 ctaacccaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc 
    1861 atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat 
    1921 ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct 
    1981 acctgacgct ttttatcgca actctctact gtttctccat acccgttttt ttggatggag 
    2041 tgaaaggagg aattcggggc aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg 
    2101 aaggcataca ggcaagaact gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc 
    2161 gcaagccgca ccgtcatgcg tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc 
    2221 cagcaagcta cggccaagat cgagcgcgac agcgtgcaac tggctccccc tgccctgccc 
    2281 gcgccatcgg ccgccgtgga gcgttcgcgt cgtctcgaac aggaggcggc aggtttggcg 
    2341 aagtcgatga ccatcgacac gcgaggaact atgacgacca agaagcgaaa aaccgccggc 
    2401 gaggacctgg caaaacaggt cagcgaggcc aagcaggccg cgttgctgaa acacacgaag 
    2461 cagcagatca aggaaatgca gctttccttg ttcgatattg cgccgtggcc ggacacgatg 
    2521 cgagcgatgc caaacgacac ggcccgctct gccctgttca ccacgcgcaa caagaaaatc 
    2581 ccgcgcgagg cgctgcaaaa caaggtcatt ttccacgtca acaaggacgt gaagatcacc 
    2641 tacaccggcg tcgagctgcg ggccgacgat gacgaactgg tgtggctgca ggtgttggag 
    2701 tacgcgaagc gcacccctat cggcgagccg atcaccttca cgttctacga gctttgccag 
    2761 gacctgggct ggtcgatcaa tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc 
    2821 ctacaggcga cggcgatgga cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg 
    2881 ctgctgcacc gcttccgcgt cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg 
    2941 atcgacgagg aaatcgtcgt gctgtttgct ggcgaccact acacgaaatt catatgggag 
    3001 aagtaccgca agctgtcgcc gacggcccga cggatgttcg actatttcag ctcgcaccgg 
    3061 gagccgtacc cgctcaagct ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc 
    3121 gtgaagaagt ggcgcgagca ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg 
    3181 gtggaacacg cctgggtcaa tgatgacctg gtgcattgca aacgctaggg ccttgtgggg 
    3241 tcagttccgg ctgggggttc agcagccacc gggccgtcga ccaattctca tgtttgacag 
    3301 cttatcatcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaacca 
    3361 ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg 
    3421 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga 
    3481 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg 
    3541 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa 
    3601 ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag 
    3661 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa 
    3721 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg 
    3781 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat 
    3841 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc 
    3901 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag 
    3961 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata 
    4021 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat 
    4081 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa 
    4141 cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta 
    4201 tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat 
    4261 tcgcgataag ctcatggagc ggcgtaaccg tcgcacagga aggacagaga aagcgcggat 
    4321 ctgggaagtg acggacagaa cggtcaggac ctggattggg gaggcggttg ccgccgctgc 
    4381 tgctgacggt gtgacgttct ctgttccggt cacaccacat acgttccgcc attcctatgc 
    4441 gatgcacatg ctgtatgccg gtataccgct gaaagttctg caaagcctga tgggacataa 
    4501 gtccatcagt tcaacggaag tctacacgaa ggtttttgcg ctggatgtgg ctgcccggca 
    4561 ccgggtgcag tttgcgatgc cggagtctga tgcggttgcg atgctgaaac aattatcctg 
    4621 agaataaatg ccttggcctt tatatggaaa tgtggaactg agtggatatg ctgtttttgt 
    4681 ctgttaaaca gagaagctgg ctgttatcca ctgagaagcg aacgaaacag tcgggaaaat 
    4741 ctcccattat cgtagagatc cgcattatta atctcaggag cctgtgtagc gtttatagga 
    4801 agtagtgttc tgtcatgatg cctgcaagcg gtaacgaaaa cgatttgaat atgccttcag 
    4861 gaacaataga aatcttcgtg cggtgttacg ttgaagtgga gcggattatg tcagcaatgg 
    4921 acagaacaac ctaatgaaca cagaaccatg atgtggtctg tccttttaca gccagtagtg 
    4981 ctcgccgcag tcgagcgaca gggcgaagcc ctcgagatcc cctgccattt ttggggtgag 
    5041 gccgttcgcg gccgaggggc gcagcccctg gggggatggg aggcccgcgt tagcggccgg 
    5101 gagggttcga gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca 
    5161 gccctggtta aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta 
    5221 gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag 
    5281 cccctcaaat gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa 
    5341 ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact 
    5401 tatccccagg cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc 
    5461 gatttgcgag gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt 
    5521 caacgccgcg ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt 
    5581 gagggccaag ttttccgcga ggtatccaca acgccggcgg ccggccgcgg tgtctcgcac 
    5641 acggcttcga cggcgtttct ggcgcgtttg cagggccata gacggccgcc agcccagcgg 
    5701 cgagggcaac cagcccggtg agcgtcggaa aggggaattt cgagtgagcg aggaagcacc 
    5761 agggaacagc acttatatat tctgcttaca cacgatgcct gaaaaaactt cccttggggt 
    5821 tatccactta tccacgggga tatttttata attatttttt ttatagtttt tagatcttct 
    5881 tttttagagc gccttgtagg cctttatcca tgctggttct agagaaggtg ttgtgacaaa 
    5941 ttgccctttc agtgtgacaa atcaccctca aatgacagtc ctgtctgtga caaattgccc 
    6001 ttaaccctgt gacaaattgc cctcagaaga agctgttttt tcacaaagtt atccctgctt 
    6061 attgactctt ttttatttag tgtgacaatc taaaaacttg tcacacttca catggatctg 
    6121 tcatggcgga aacagcggtt atcaatcaca agaaacgtaa aaatagcccg cgaatcgtcc 
    6181 agtcaaacga cctcactgag gcggcatata gtctctcccg ggatcaaaaa cgtatgctgt 
    6241 atctgttcgt tgaccagatc agaaaatctg atggcaccct acaggaacat gacggtatct 
    6301 gcgagatcca tgttgctaaa tatgctgaaa tattcggatt gacctctgcg gaagccagta 
    6361 aggatatacg gcaggcattg aagagtttcg cggggaagga agtggttttt tatcgccctg 
    6421 aagaggatgc cggcgatgaa aaaggctatg aatcttttcc ttggtttatc aaacgtgcgc 
    6481 acagtccatc cagagggctt tacagtgtac atatcaaccc atatctcatt cccttcttta 
    6541 tcgggttaca gaaccggttt acgcagtttc ggcttagtga aacaaaagaa atcaccaatc 
    6601 cgtatgccat gcgtttatac gaatccctgt gtcagtatcg taagccggat ggctcaggca 
    6661 tcgtctctct gaaaatcgac tggatcatag agcgttacca gctgcctcaa agttaccagc 
    6721 gtatgcctga cttccgccgc cgcttcctgc aggtctgtgt taatgagatc aacagcagaa 
    6781 ctccaatgcg cctctcatac attgagaaaa agaaaggccg ccagacgact catatcgtat 
    6841 tttccttccg cgatatcact tccatgacga caggatagtc tgagggttat ctgtcacaga 
    6901 tttgagggtg gttcgtcaca tttgttctga cctactgagg gtaatttgtc acagttttgc 
    6961 tgtttccttc agcctgcatg gattttctca tactttttga actgtaattt ttaaggaagc 
    7021 caaatttgag ggcagtttgt cacagttgat ttccttctct ttcccttcgt catgtgacct 
    7081 gatatcgggg gttagttcgt catcattgat gagggttgat tatcacagtt tattactctg 
    7141 aattggctat ccgcgtgtgt acctctacct ggagtttttc ccacggtgga tatttcttct 
    7201 tgcgctgagc gtaagagcta tctgacagaa cagttcttct ttgcttcctc gccagttcgc 
    7261 tcgctatgct cggttacacg gctgcggcga gcgctagtga taataagtga ctgaggtatg 
    7321 tgctcttctt atctcctttt gtagtgttgc tcttatttta aacaactttg cggttttttg 
    7381 atgactttgc gattttgttg ttgctttgca gtaaattgca agatttaata aaaaaacgca 
    7441 aagcaatgat taaaggatgt tcagaatgaa actcatggaa acacttaacc agtgcataaa 
    7501 cgctggtcat gaaatgacga aggctatcgc cattgcacag tttaatgatg acagcccgga 
    7561 agcgaggaaa ataacccggc gctggagaat aggtgaagca gcggatttag ttggggtttc 
    7621 ttctcaggct atcagagatg ccgagaaagc agggcgacta ccgcacccgg atatggaaat 
    7681 tcgaggacgg gttgagcaac gtgttggtta tacaattgaa caaattaatc atatgcgtga 
    7741 tgtgtttggt acgcgattgc gacgtgctga agacgtattt ccaccggtga tcggggttgc 
    7801 tgcccataaa ggtggcgttt acaaaacctc agtttctgtt catcttgctc aggatctggc 
    7861 tctgaagggg ctacgtgttt tgctcgtgga aggtaacgac ccccagggaa cagcctcaat 
    7921 gtatcacgga tgggtaccag atcttcatat tcatgcagaa gacactctcc tgcctttcta 
    7981 tcttggggaa aaggacgatg tcacttatgc aataaagccc acttgctggc cggggcttga 
    8041 cattattcct tcctgtctgg ctctgcaccg tattgaaact gagttaatgg gcaaatttga 
    8101 tgaaggtaaa ctgcccaccg atccacacct gatgctccga ctggccattg aaactgttgc 
    8161 tcatgactat gatgtcatag ttattgacag cgcgcctaac ctgggtatcg gcacgattaa 
    8221 tgtcgtatgt gctgctgatg tgctgattgt tcccacgcct gctgagttgt ttgactacac 
    8281 ctccgcactg cagtttttcg atatgcttcg tgatctgctc aagaacgttg atcttaaagg 
    8341 gttcgagcct gatgtacgta ttttgcttac caaatacagc aatagtaatg gctctcagtc 
    8401 cccgtggatg gaggagcaaa ttcgggatgc ctggggaagc atggttctaa aaaatgttgt 
    8461 acgtgaaacg gatgaagttg gtaaaggtca gatccggatg agaactgttt ttgaacaggc 
    8521 cattgatcaa cgctcttcaa ctggtgcctg gagaaatgct ctttctattt gggaacctgt 
    8581 ctgcaatgaa attttcgatc gtctgattaa accacgctgg gagattagat aatgaagcgt 
    8641 gcgcctgtta ttccaaaaca tacgctcaat actcaaccgg ttgaagatac ttcgttatcg 
    8701 acaccagctg ccccgatggt ggattcgtta attgcgcgcg taggagtaat ggctcgcggt 
    8761 aatgccatta ctttgcctgt atgtggtcgg gatgtgaagt ttactcttga agtgctccgg 
    8821 ggtgatagtg ttgagaagac ctctcgggta tggtcaggta atgaacgtga ccaggagctg 
    8881 cttactgagg acgcactgga tgatctcatc ccttcttttc tactgactgg tcaacagaca 
    8941 ccggcgttcg gtcgaagagt atctggtgtc atagaaattg ccgatgggag tcgccgtcgt 
    9001 aaagctgctg cacttaccga aagtgattat cgtgttctgg ttggcgagct ggatgatgag 
    9061 cagatggctg cattatccag attgggtaac gattatcgcc caacaagtgc ttatgaacgt 
    9121 ggtcagcgtt atgcaagccg attgcagaat gaatttgctg gaaatatttc tgcgctggct 
    9181 gatgcggaaa atatttcacg taagattatt acccgctgta tcaacaccgc caaattgcct 
    9241 aaatcagttg ttgctctttt ttctcacccc ggtgaactat ctgcccggtc aggtgatgca 
    9301 cttcaaaaag cctttacaga taaagaggaa ttacttaagc agcaggcatc taaccttcat 
    9361 gagcagaaaa aagctggggt gatatttgaa gctgaagaag ttatcactct tttaacttct 
    9421 gtgcttaaaa cgtcatctgc atcaagaact agtttaagct cacgacatca gtttgctcct 
    9481 ggagcgacag tattgtataa gggcgataaa atggtgctta acctggacag gtctcgtgtt 
    9541 ccaactgagt gtatagagaa aattgaggcc attcttaagg aacttgaaaa gccagcaccc 
    9601 tgatgcgacc acgttttagt ctacgtttat ctgtctttac ttaatgtcct ttgttacagg 
    9661 ccagaaagca taactggcct gaatattctc tctgggccca ctgttccact tgtatcgtcg 
    9721 gtctgataat cagactggga ccacggtccc actcgtatcg tcggtctgat tattagtctg 
    9781 ggaccacggt cccactcgta tcgtcggtct gattattagt ctgggaccac ggtcccactc 
    9841 gtatcgtcgg tctgataatc agactgggac cacggtccca ctcgtatcgt cggtctgatt 
    9901 attagtctgg gaccatggtc ccactcgtat cgtcggtctg attattagtc tgggaccacg 
    9961 gtcccactcg tatcgtcggt ctgattatta gtctggaacc acggtcccac tcgtatcgtc 
   10021 ggtctgatta ttagtctggg accacggtcc cactcgtatc gtcggtctga ttattagtct 
   10081 gggaccacga tcccactcgt gttgtcggtc tgattatcgg tctgggacca cggtcccact 
   10141 tgtattgtcg atcagactat cagcgtgaga ctacgattcc atcaatgcct gtcaagggca 
   10201 agtattgaca tgtcgtcgta acctgtagaa cggagtaacc tcggtgtgcg gttgtatgcc 
   10261 tgctgtggat tgctgctgtg tcctgcttat ccacaacatt ttgcgcacgg ttatgtggac 
   10321 aaaatacctg gttacccagg ccgtgccggc acgttaaccg ggctgcatcc gatgcaagtg 
   10381 tgtcgctgtc gacgagctcg cgagctcgga catgaggttg ccccgtattc agtgtcgctg 
   10441 atttgtattg tctgaagttg tttttacgtt aagttgatgc agatcaatta atacgatacc 
   10501 tgcgtcataa ttgattattt gacgtggttt gatggcctcc acgcacgttg tgatatgtag 
   10561 atgataatca ttatcacttt acgggtcctt tccggtgatc cgacaggtta cggggcggcg 
   10621 acctcgcggg ttttcgctat ttatgaaaat tttccggttt aaggcgtttc cgttcttctt 
   10681 cgtcataact taatgttttt atttaaaata ccctctgaaa agaaaggaaa cgacaggtgc 
   10741 tgaaagcgag ctttttggcc tctgtcgttt cctttctctg tttttgtccg tggaatgaac 
   10801 aatggaagtc cgagctcatc gctaataact tcgtatagca tacattatac gaagttatat 
   10861 tcgatgcggc ctagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct 
   10921 caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt 
   10981 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 
   11041 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca 
   11101 gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc 
   11161 ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga 
   11221 tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc 
   11281 gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca 
   11341 gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca 
   11401 tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt 
   11461 tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg 
   11521 cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat 
   11581 gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa 
   11641 cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga 
   11701 tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc 
   11761 cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa 
   11821 tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca 
   11881 gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg 
   11941 ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca 
   12001 cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg 
   12061 gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa 
   12121 aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc 
   12181 attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt 
   12241 gcatgtaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg 
   12301 ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg 
   12361 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg 
   12421 gcgtagagga tcgagatcga tctcgatccc gcgaaattaa tacgactcac tata