pGPS2.1

At a Glance

Image of pGPS2.1

Type	Genome Priming System
Origin of Replication	R6K
Copy #
Markers	tetracycline, chloramphenicol
Link to Sequence	NEB
Comments	The GPS-2 System includes two Transprimer transposons-one encodes kanamycin resistance and the other encodes chloramphenicol resistance.
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History

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Sources

pGPS2.1 is isolated from E. coli BW23474

References

New England Biolabs

Sequence

      1  GGTACCCTGT GAATGCGCAA ACCAACCCTT GGCAGAACAT ATCCATCGCG 
     51  TCCGCCATCT CCAGCAGCCG CACGCGGCGC ATCTCGGGCA GCGTTGGGTC 
    101  CTGGCCACGG GTGCGCATGA TCGTGCTCCT GTCGTTGAGG ACCCGGCTAG 
    151  GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG 
    201  CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC 
    251  ATGAATGGTC TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT 
    301  CAGCGCCCTG CACCATTATG TTCCGGATCT ATGTCGGGTG CGGAGAAAGA 
    351  GGTAATGAAA TGGCAGATCC CTGGCTTGTT GTCCACAACC GTTAAACCTT 
    401  AAAAGCTTTA AAAGCCTTAT ATATTCTTTT TTTTCTTATA AAACTTAAAA 
    451  CCTTAGAGGC TATTTAAGTT GCTGATTTAT ATTAATTTTA TTGTTCAAAC 
    501  ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTT AGCCATGAGA 
    551  GCTTAGTACG TTAGCCATGA GGGTTTAGTT CGTTAAACAT GAGAGCTTAG 
    601  TACGTTAAAC ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTA 
    651  CTATCAACAG GTTGAACTGC TGATCTTCGG ATCTATGTCG GGTGCGGAGA 
    701  AAGAGGTAAT GAAATGGCAG ATCCCTGGCT TGTTGTCCAC AACCGTTAAA 
    751  CCTTAAAAGC TTTAAAAGCC TTATATATTC TTTTTTTTCT TATAAAACTT 
    801  AAAACCTTAG AGGCTATTTA AGTTGCTGAT TTATATTAAT TTTATTGTTC 
    851  AAACATGAGA GCTTAGTACG TGAAACATGA GAGCTTAGTA CGTTAGCCAT 
    901  GAGAGCTTAG TACGTTAGCC ATGAGGGTTT AGTTCGTTAA ACATGAGAGC 
    951  TTAGTACGTT AAACATGAGA GCTTAGTACG TGAAACATGA GAGCTTAGTA 
   1001  CGTACTATCA ACAGGTTGAA CTGCTGATCT TCGGATCTAT GTCGGGTGCG 
   1051  GAGAAAGAGG TAATGAAATG GCATCCGGAT CTGCATCGCA GGATGCTGCT 
   1101  GGCTACCCTG TGGAACACCT ACATCTGTAT TAACGAAGCA TTATTGAAGC 
   1151  ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA 
   1201  GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 
   1251  CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG 
   1301  CGTATCACGA GGCCCTTTCG TCTTCAAGAA TTCTCATGTT TGACAGCTTA 
   1351  TCATCGATAA GCTTTAATGC GGTAGTTTAT CACAGTTAAA TTGCTAACGC 
   1401  AGTCAGGCAC CGTGTATGAA ATCTAACAAT GCGCTCATCG TCATCCTCGG 
   1451  CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG CCGGTACTGC 
   1501  CGGGCCTCTT GCGGGATATC GTCCATTCCG ACAGCATCGC CAGTCACTAT 
   1551  GGCGTGCTGC TAGCGCTATA TGCGTTGATG CAATTTCTAT GCGCACCCGT 
   1601  TCTCGGAGCA CTGTCCGACC GCTTTGGCCG CCGCCCAGTC CTGCTCGCTT 
   1651  CGCTACTTGG AGCCACTATC GACTACGCGA TCATGGCGAC CACACCCGTC 
   1701  CTGTGGATCC TCTACGCCGG ACGCATCGTG GCCGGCATCA CCGGCGCCAC 
   1751  AGGTGCGGTT GCTGGCGCCT ATATCGCCGA CATCACCGAT GGGGAAGATC 
   1801  GGGCTCGCCA CTTCGGGCTC ATGAGCGCTT GTTTCGGCGT GGGTATGGTG 
   1851  GCAGGCCCCG TGGCCGGGGG ACTGTTGGGC GCCATCTCCT TGCATGCACC 
   1901  ATTCCTTGCG GCGGCGGTGC TCAACGGCCT CAACCTACTA CTGGGCTGCT 
   1951  TCCTAATGCA GGAGTCGCAT AAGGGAGAGC GTCGACCGAT GCCCTTGAGA 
   2001  GCCTTCAACC CAGTCAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT 
   2051  CGCCGCACTT ATGACTGTCT TCTTTATCAT GCAACTCGTA GGACAGGTGC 
   2101  CGGCAGCGCT CTGGGTCATT TTCGGCGAGG ACCGCTTTCG CTGGAGCGCG 
   2151  ACGATGATCG GCCTGTCGCT TGCGGTATTC GGAATCTTGC ACGCCCTCGC 
   2201  TCAAGCCTTC GTCACTGGTC CCGCCACCAA ACGTTTCGGC GAGAAGCAGG 
   2251  CCATTATCGC CGGCATGGCG GCCGACGCGC TGGGCTACGT CTTGCTGGCG 
   2301  TTCGCGACGC GAGGCTGGAT GGCCTTCCCC ATTATGATTC TTCTCGCTTC 
   2351  CGGCGGCATC GGGATGCCCG CGTTGCAGGC CATGCTGTCC AGGCAGGTAG 
   2401  ATGACGACCA TCAGGGACAG CTTCAAGGAT CGCTCGCGGC TCTTACCAGC 
   2451  CTAACTTCGA TCATTGGACC GCTGATCGTC ACGGCGATTT ATGCCGCCTC 
   2501  GGCGAGCACA TGGAACGGGT TGGCATGGAT TGTAGGCGCC GCCCTATACC 
   2551  TTGTCTGCCT CCCCGCGTTG CGTCGCGGTG CATGGAGCCG GGCCACCTCG 
   2601  ACCTGAATGG AAGCCGGCGG CACCTCGCTA ACGGATTCAC CACTCCAAGA 
   2651  ATTGGAGCCA ATCAATTCTT GCGGAGAACT GTGAATGCGC AAACCAACCC 
   2701  TTGGCAGAAC ATATCCATCG CGTCCGCCAT CTCCAGCAGC CGCACGCGGC 
   2751  GCATCTCGGG CAGCGTTGGG TCCTGGGCTG GCATTGACCC TGAGTGATTT 
   2801  TTCTCTGGTC CCGCCGCATC CATACCGCCA GTTGTTTACC CTCACAACGT 
   2851  TCCAGTAACC GGGCATGTTC ATCATCAGTA ACCCGTATCG TGAGCATCCT 
   2901  CTCTCGTTTC ATCGGTATCA TTACCCCCAT GAACAGAAAT CCCCCTTACA 
   2951  CGGAGGCATC AGTGACCAAA CAGGAAAAAA CCGCCCTTAA CATGGCCCGC 
   3001  TTTATCAGAA GCCAGACATT AACGCTTCTG GAGAAACTCA ACGAGCTGGA 
   3051  CGCGGATGAA CAGGCAGAGC TCTTACTGTC ATGCCATCCG TATGTGGGCG 
   3101  GACAATAAAG TCTTAAACTG AACAAAATAG ATCTAAACTA TGACAATAAA 
   3151  GTCTTAAACT AGACAGAATA GTTGTAAACT GAAATCAGTC CAGTTATGCT 
   3201  GTGAAAAAGC ATACTGGACT TTTGTTATGG CTAAAGCAAA CTCTTCATTT 
   3251  TCTGAAGTGC AAATTGCCCG TCGTATTAAA GAGGGGCGTG GGGTCGACGC 
   3301  GGCCGCTAAC TATAACGGTC CTAAGGTAGC GAGTTTAAAC GATATCGGAT 
   3351  CCAGGCGTTT AAGGGCACCA ATAACTGCCT TAAAAAAATT ACGCCCCGCC 
   3401  CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCCGACAT 
   3451  GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC 
   3501  ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA 
   3551  GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC 
   3601  AGGGATTGGC TGAGACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA 
   3651  TAGGCCAGGT TTTCACCGTA ACACGCCACA TCTTGCGAAT ATATGTGTAG 
   3701  AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT 
   3751  CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC 
   3801  ACCAGCTCAC CGTCTTTCAT TGCCATACGG AATTCCGGAT GAGCATTCAT 
   3851  CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT 
   3901  TCTTTACGGT CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA 
   3951  TAGGTACATT GAGCAACTGA CTGAAATGCC TCAAAATGTT CTTTACGATG 
   4001  CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT TTCTCCATTT 
   4051  TAGCTTCCTT AGCTCCTGAA AATCTCGATA ACTCAAAAAA TACGCCCGGT 
   4101  AGTGATCTTA TTTCATTATG GTGAAAGTTG GAACCTCTTA CGTGCCGATC 
   4151  AACGTCTCAT TTTCGCCAAA AGTTGGCCCA GGGCTTCCCG GTATCAACAG
   4201  GGACACCAGG ATTTATTTAT TCTGCGAAGT GATCTTCCGT CACAGGTATG 
   4251  GATCCGATAT CATTTAAATC TAGGGATAAC AGGGTAATAC TAGTGTCGAC  
   4301  CAACCAGATA AGTGAAATCT AGTTCCAAAC TATTTTGTCA TTTTTAATTT 
   4351  TCGTATTAGC TTACGACGCT ACACCCAGTT CCCATCTATT TTGTCACTCT
   4401  TCCCTAAATA ATCCTTAAAA ACTCCATTTC CACCCCTCCC AGTTCCCAAC 
   4451  TATTTTGTCC GCCCACACCG TAAGATGCTT TTCTGTGACT