HB101: Difference between revisions

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| Genotype
| Genotype
| Different sources have different genotypes for HB101. The genotype listed below is from the Coli Genetics Stock Center.  
| Different sources have different genotypes for HB101. The genotype listed below is from the Coli Genetics Stock Center.  
| F<sup>-</sup> Lambda<sup>-</sup> araC14 leuB6(Am) DE(gpt-proA)62 lacY1 glnX44(AS) galK2(Oc) recA13 rpsL20(strR) xylA5 mtl-1 thiE1 hsdS20(r<sub>B</sub><sup>-</sup>, m<sub>B</sub><sup>-</sup>)
<br>F<sup>-</sup> Lambda<sup>-</sup> araC14 leuB6(Am) DE(gpt-proA)62 lacY1 glnX44(AS) galK2(Oc) recA13 rpsL20(strR) xylA5 mtl-1 thiE1 hsdS20(r<sub>B</sub><sup>-</sup>, m<sub>B</sub><sup>-</sup>)<br><br>
|
NEB: includes DE(mcrC-mrr)<br>
| NEB: includes DE(mcrC-mrr)
Promega (and others): proA2
| Promega (and others): proA2
|-
|-
| Phenotype
| Phenotype
| Streptomycin resistance
| Streptomycin resistance<br>
| Lacks the K-12 restriction-modification system
Lacks the K-12 restriction-modification system
|-
|-
| Sources
| Sources
| [https://bccm.belspo.be/catalogues/genecorner-host-details?NUM=LMBP%20684 LMBP 684]
| [https://bccm.belspo.be/catalogues/genecorner-host-details?NUM=LMBP%20684 LMBP 684]<br>
| [http://cgsc.biology.yale.edu/Strain.php?ID=130465 CGSC 12554
[http://cgsc.biology.yale.edu/Strain.php?ID=130465 CGSC 12554]
|-
|-
| References
| References
| Boyer, H.W. and Roulland-Dussoix, D. (1969) J. Mol. Biol. 41, 459.
| Boyer, H.W. and Roulland-Dussoix, D. (1969) J. Mol. Biol. 41, 459.
| Smith, M., Lorow, D., and Jessee, J. (1989) FOCUS 11, 56.
<br>Smith, M., Lorow, D., and Jessee, J. (1989) FOCUS 11, 56.
| Lacks S and Greenberg JR (1977) J Mol Biol 114:153.
<br>Lacks S and Greenberg JR (1977) J Mol Biol 114:153.
|}
|}



Revision as of 12:17, 16 October 2020

Name KM5219
Synonyms
Genotype Different sources have different genotypes for HB101. The genotype listed below is from the Coli Genetics Stock Center.


F- Lambda- araC14 leuB6(Am) DE(gpt-proA)62 lacY1 glnX44(AS) galK2(Oc) recA13 rpsL20(strR) xylA5 mtl-1 thiE1 hsdS20(rB-, mB-)

NEB: includes DE(mcrC-mrr)
Promega (and others): proA2

Phenotype Streptomycin resistance

Lacks the K-12 restriction-modification system

Sources LMBP 684

CGSC 12554

References Boyer, H.W. and Roulland-Dussoix, D. (1969) J. Mol. Biol. 41, 459.


Smith, M., Lorow, D., and Jessee, J. (1989) FOCUS 11, 56.
Lacks S and Greenberg JR (1977) J Mol Biol 114:153.

Notes