IM01B: Difference between revisions
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Created page with "{| class="wikitable" border="1" |- | Name | IM01B |- | Synonyms | |- | Genotype | mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL..." |
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| Sources | | Sources | ||
| [http://bccm.belspo.be/catalogues/files/ | | [http://bccm.belspo.be/catalogues/files/genecorner-hosts/IM01B.pdf LMBP 9581] | ||
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| References | | References | ||
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[[Category:BCCM/ | [[Category:BCCM/GeneCorner]][[Category:Strains]] | ||
Revision as of 13:27, 16 August 2018
| Name | IM01B |
| Synonyms | |
| Genotype | mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG Δdcm ΩPhelp-hsdMS (CC1-2) ΩPN25-hsdS (CC1-1) |
| Phenotype | streptomycin resistant |
| Sources | LMBP 9581 |
| References | PMID:26015493 |
Notes
This bacterial host strain was derived from E. coli K12 DH10B by deleting the dcm gene encoding cytosine methylation. Additionally, the hsdMS genes encoding methylase and specificity genes (from Staphylococcus aureus clonal complex 1 - MW2) were introduced into the chromosome at neutral locations via recombineering.
The strain can be transformed efficiently with large plasmids due to deoR (nupG) mutation.
Plasmids isolated from this E. coli strain transform S. aureus clonal complex 1 - MW2 at high efficiency.