PMID:3511029: Difference between revisions
New PMID: Page! |
Fill PMID: Page! |
||
| Line 1: | Line 1: | ||
{{RightTOC}} | |||
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.183549.F539a737fa8d8e--> | |||
<!-- | |||
****************************************************************************************** | |||
* | |||
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | |||
* | |||
****************************************************************************************** --> | |||
{| id="F539a737fa8d8e" class=" tableEdit PMID_info_table" | |||
|- | |||
!align=left |Citation | |||
|| | |||
'''Salowe, SP and Stubbe, J''' (1986) Cloning, overproduction, and purification of the B2 subunit of ribonucleoside-diphosphate reductase. ''J. Bacteriol.'' '''165''':363-6 | |||
|- | |||
!align=left |Abstract | |||
|| | |||
The nrdB gene, which encodes the B2 subunit of Escherichia coli ribonucleotide reductase (EC 1.17.4.1), was cloned into multicopy plasmid pSPS2. This vector, which contains the pL promoter of bacteriophage lambda and the tetracycline resistance gene of pBR322, was transformed into a lysogenic host with a thermolabile repressor. In the newly constructed strain, subunit B2 constituted approximately 25% of the soluble protein after heat induction, an overproduction of several hundredfold relative to the wild-type strain. Purification to homogeneity of the overproduced protein was accomplished by using DEAE and quaternary aminoethyl ion-exchange resins. | |||
|- | |||
!align=left |Links | |||
|| | |||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3511029 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC214425 PMC214425] | |||
|- | |||
!align=left |Keywords | |||
|| | |||
Amino Acid Sequence; Cloning, Molecular; Escherichia coli/enzymology; Gene Expression Regulation; Macromolecular Substances; Ribonucleoside Diphosphate Reductase/biosynthesis; Ribonucleoside Diphosphate Reductase/genetics; Ribonucleoside Diphosphate Reductase/isolation & purification; Ribonucleotide Reductases/genetics | |||
|- class="tableEdit_footer" | |||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.183549.F539a737fa8d8e&page=183549&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> || | |||
|} | |||
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.183549.F539a737fa8d8e--> | |||
==Significance {{HelpIcon|Help:Literature}} == | |||
{{LitSignificance}} | |||
== Useful Materials and Methods {{HelpIcon|Help:Literature}}== | |||
{{LitMaterials}} | |||
==Annotations {{HelpIcon|Help:Literature}}== | |||
<annotationlinks/> | |||
==EcoliWiki Links {{HelpIcon|Help:Literature}} == | |||
{{LitLinks}} | |||
==See also== | |||
==References== | |||
{{RefHelp}} | |||
<references/> | |||
Latest revision as of 03:44, 13 June 2014
| Citation |
Salowe, SP and Stubbe, J (1986) Cloning, overproduction, and purification of the B2 subunit of ribonucleoside-diphosphate reductase. J. Bacteriol. 165:363-6 |
|---|---|
| Abstract |
The nrdB gene, which encodes the B2 subunit of Escherichia coli ribonucleotide reductase (EC 1.17.4.1), was cloned into multicopy plasmid pSPS2. This vector, which contains the pL promoter of bacteriophage lambda and the tetracycline resistance gene of pBR322, was transformed into a lysogenic host with a thermolabile repressor. In the newly constructed strain, subunit B2 constituted approximately 25% of the soluble protein after heat induction, an overproduction of several hundredfold relative to the wild-type strain. Purification to homogeneity of the overproduced protein was accomplished by using DEAE and quaternary aminoethyl ion-exchange resins. |
| Links | |
| Keywords |
Amino Acid Sequence; Cloning, Molecular; Escherichia coli/enzymology; Gene Expression Regulation; Macromolecular Substances; Ribonucleoside Diphosphate Reductase/biosynthesis; Ribonucleoside Diphosphate Reductase/genetics; Ribonucleoside Diphosphate Reductase/isolation & purification; Ribonucleotide Reductases/genetics |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.