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| | !align=left |Citation | ||
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'''No author listed''' (2011) High-flux isobutanol production using engineered Escherichia coli: a bioreactor study with in situ product removal. ''Appl. Microbiol. Biotechnol.'' '''90''':1681-90 | |||
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| | !align=left |Abstract | ||
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Promising approaches to produce higher alcohols, e.g., isobutanol, using Escherichia coli have been developed with successful results. Here, we translated the isobutanol process from shake flasks to a 1-L bioreactor in order to characterize three E. coli strains. With in situ isobutanol removal from the bioreactor using gas stripping, the engineered E. coli strain (JCL260) produced more than 50 g/L in 72 h. In addition, the isobutanol production by the parental strain (JCL16) and the high isobutanol-tolerant mutant (SA481) were compared with JCL260. Interestingly, we found that the isobutanol-tolerant strain in fact produced worse than either JCL16 or JCL260. This result suggests that in situ product removal can properly overcome isobutanol toxicity in E. coli cultures. The isobutanol productivity was approximately twofold and the titer was 9% higher than n-butanol produced by Clostridium in a similar integrated system. | |||
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!align=left |Links | |||
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=21547458 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3094657 PMC3094657] | |||
Online version:[http://dx.doi.org/10.1007/s00253-011-3173-y 10.1007/s00253-011-3173-y] | Online version:[http://dx.doi.org/10.1007/s00253-011-3173-y 10.1007/s00253-011-3173-y] | ||
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!align=left |Keywords | |||
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Bioreactors/microbiology; Butanols/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Genetic Engineering; Industrial Microbiology/methods | |||
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==Significance {{HelpIcon|Help:Literature}} == | ==Significance {{HelpIcon|Help:Literature}} == | ||
{{LitSignificance}} | {{LitSignificance}} | ||
Revision as of 19:13, 1 September 2013
| Citation |
No author listed (2011) High-flux isobutanol production using engineered Escherichia coli: a bioreactor study with in situ product removal. Appl. Microbiol. Biotechnol. 90:1681-90 |
|---|---|
| Abstract |
Promising approaches to produce higher alcohols, e.g., isobutanol, using Escherichia coli have been developed with successful results. Here, we translated the isobutanol process from shake flasks to a 1-L bioreactor in order to characterize three E. coli strains. With in situ isobutanol removal from the bioreactor using gas stripping, the engineered E. coli strain (JCL260) produced more than 50 g/L in 72 h. In addition, the isobutanol production by the parental strain (JCL16) and the high isobutanol-tolerant mutant (SA481) were compared with JCL260. Interestingly, we found that the isobutanol-tolerant strain in fact produced worse than either JCL16 or JCL260. This result suggests that in situ product removal can properly overcome isobutanol toxicity in E. coli cultures. The isobutanol productivity was approximately twofold and the titer was 9% higher than n-butanol produced by Clostridium in a similar integrated system. |
| Links |
PubMed PMC3094657 Online version:10.1007/s00253-011-3173-y |
| Keywords |
Bioreactors/microbiology; Butanols/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Genetic Engineering; Industrial Microbiology/methods |
| edit table |
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