Phage f1: Difference between revisions
Saleksander (talk | contribs) |
Saleksander (talk | contribs) |
||
| Line 20: | Line 20: | ||
==== Packaging ==== | ==== Packaging ==== | ||
The phage envelope consists of about 2700 copies of the major coat protein encoded by [[Phage_f1_VIII:Gene_Product(s)| pVIII]]. <ref name='PMID:9636697'/> As with most filamentous phages, there is no defined limit on the amount of DNA that can be packaged into the phage coat (contrast to phages with set capsid size, such as [[Bacteriophage_λ| phage λ]]. | The phage envelope consists of about 2700 copies of the major coat protein encoded by [[Phage_f1_VIII:Gene_Product(s)| pVIII]]. <ref name='PMID:9636697'/> As with most filamentous phages, there is no defined limit on the amount of DNA that can be packaged into the phage coat (contrast to phages with set capsid size, such as [[Bacteriophage_λ| phage λ]]) although larger inserts are naturally selected against. <ref name='LIB:The Bacteriophages'/> | ||
==== Lysis ==== | ==== Lysis ==== | ||
Revision as of 17:05, 28 June 2011
Bacteriophage f1 is closely related to Phage M13
The full sequence is available from NCBI at GenBank: J02448.1
Phage f1, a ssRNA phage, should not be confused with phage F1, a ssDNA phage, but f1 may also refer to a ssDNA phage.
Morphology
Phage f1 is a filamentous (rod-shaped) ssDNA phage, with a molecular mass of about 1.6x107 Da; by weight it is 11.3 percent DNA. [1] [2] The flexible rods are about 8500 Å (850 nm) long, and depending on the staining technique, appear to be 43 or 63 Å wide. [2] The thousands of identical major coat proteins that make up the sheath are slightly curved, enlongated proteins about 70 Å by 10 Å arranged in an α-helix. [3] [4] These sheath proteins slightly overlap and are oriented just slightly off of perpendicular to the length of the filament; they are interdigitated and slope radially to form a fishscale-like envelope for the DNA core. [3] The ends of the filament are sealed with several minor protein caps
Genome Organization
6407 bp of ss-DNA, circular
10 genes encoded; generally referred to by Roman numerals I-X as listed on the f1 Gene page. Genes are in the order: II (X), V, VII, IX, VIII, III, VI, I, IV, intergenic region. [5]
Life cycle
Adsorption and Injection
f1 is male specific; it only infects Hfr or F+ strains of E. coli. [2]
DNA replication
Packaging
The phage envelope consists of about 2700 copies of the major coat protein encoded by pVIII. [6] As with most filamentous phages, there is no defined limit on the amount of DNA that can be packaged into the phage coat (contrast to phages with set capsid size, such as phage λ) although larger inserts are naturally selected against. [5]
Lysis
This phage does not lyse its host, but can extrude many copies of itself throughout the life of the host cell.
See also
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Endemann, H & Model, P (1995) Location of filamentous phage minor coat proteins in phage and in infected cells. J. Mol. Biol. 250 496-506 PubMed
- ↑ 2.0 2.1 2.2 ZINDER, ND et al. (1963) F1, A ROD-SHAPED MALE-SPECIFIC BACTERIOPHAGE THAT CONTAINS DNA. Virology 20 638-40 PubMed
- ↑ 3.0 3.1 Straus, SK et al. (2011) Consensus structure of Pf1 filamentous bacteriophage from X-ray fibre diffraction and solid-state NMR. Eur. Biophys. J. 40 221-34 PubMed
- ↑ Marvin, DA (1998) Filamentous phage structure, infection and assembly. Curr. Opin. Struct. Biol. 8 150-8 PubMed
- ↑ 5.0 5.1 Cite error: Invalid
<ref>tag; no text was provided for refs namedLIB:The Bacteriophages - ↑ Haigh, NG & Webster, RE (1998) The major coat protein of filamentous bacteriophage f1 specifically pairs in the bacterial cytoplasmic membrane. J. Mol. Biol. 279 19-29 PubMed