Phage f1: Difference between revisions
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{{Note| Phage f1, a ssRNA phage, should not be confused with phage F1, a ssDNA phage, but f1 may also refer to a ssDNA phage.}} | {{Note| Phage f1, a ssRNA phage, should not be confused with phage F1, a ssDNA phage, but f1 may also refer to a ssDNA phage.}} | ||
== Morphology == | == Morphology == | ||
Phage f1 is a filamentous (rod-shaped) ssDNA phage, with a molecular mass of about 1.6x10<sup>7</sup> Da; by weight it is 11.3 percent DNA <ref name='PMID:7616570'/> <ref name='PMID:14065763'/> | Phage f1 is a filamentous (rod-shaped) ssDNA phage, with a molecular mass of about 1.6x10<sup>7</sup> Da; by weight it is 11.3 percent DNA. <ref name='PMID:7616570'/> <ref name='PMID:14065763'/> The flexible rods are about 8500 Å (850 nm) long, and depending on the staining technique, appear to be 43 or 63 Å wide. <ref name='PMID:14065763'/> The thousands of identical major coat proteins that make up the sheath are slightly curved, enlongated proteins about 70 Å by 10 Å arranged in an α-helix. <ref name='PMID:21082179'/> <ref name='PMID:9631287'/> These sheath proteins slightly overlap and are oriented just slightly off of perpendicular to the length of the filament; they are interdigitated and slope radially to form a fishscale-like envelope for the DNA core. <ref name='PMID:21082179'/> The ends of the filament are sealed with several minor protein caps | ||
== Genome Organization == | == Genome Organization == | ||
Revision as of 16:34, 28 June 2011
Bacteriophage f1 is closely related to Phage M13
The full sequence is available from NCBI at GenBank: J02448.1
Phage f1, a ssRNA phage, should not be confused with phage F1, a ssDNA phage, but f1 may also refer to a ssDNA phage.
Morphology
Phage f1 is a filamentous (rod-shaped) ssDNA phage, with a molecular mass of about 1.6x107 Da; by weight it is 11.3 percent DNA. [1] [2] The flexible rods are about 8500 Å (850 nm) long, and depending on the staining technique, appear to be 43 or 63 Å wide. [2] The thousands of identical major coat proteins that make up the sheath are slightly curved, enlongated proteins about 70 Å by 10 Å arranged in an α-helix. [3] [4] These sheath proteins slightly overlap and are oriented just slightly off of perpendicular to the length of the filament; they are interdigitated and slope radially to form a fishscale-like envelope for the DNA core. [3] The ends of the filament are sealed with several minor protein caps
Genome Organization
6407 bp of ss-DNA, circular
10 genes encoded; generally referred to by Roman numerals I-X as listed on the f1 Gene page.
Life cycle
Adsorption and Injection
f1 is male specific; it only infects Hfr or F+ strains of E. coli. [2]
DNA replication
Packaging
The phage envelope consists of about 2700 copies of the major coat protein encoded by pVIII. [5]
Lysis
This phage does not lyse its host, but can extrude many copies of itself throughout the life of the host cell.
See also
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Endemann, H & Model, P (1995) Location of filamentous phage minor coat proteins in phage and in infected cells. J. Mol. Biol. 250 496-506 PubMed
- ↑ 2.0 2.1 2.2 ZINDER, ND et al. (1963) F1, A ROD-SHAPED MALE-SPECIFIC BACTERIOPHAGE THAT CONTAINS DNA. Virology 20 638-40 PubMed
- ↑ 3.0 3.1 Straus, SK et al. (2011) Consensus structure of Pf1 filamentous bacteriophage from X-ray fibre diffraction and solid-state NMR. Eur. Biophys. J. 40 221-34 PubMed
- ↑ Marvin, DA (1998) Filamentous phage structure, infection and assembly. Curr. Opin. Struct. Biol. 8 150-8 PubMed
- ↑ Haigh, NG & Webster, RE (1998) The major coat protein of filamentous bacteriophage f1 specifically pairs in the bacterial cytoplasmic membrane. J. Mol. Biol. 279 19-29 PubMed