Phage f1: Difference between revisions

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{{Note| Phage f1, a ssRNA phage, should not be confused with phage F1, a ssDNA phage, but f1 may also refer to a ssDNA phage.}}
{{Note| Phage f1, a ssRNA phage, should not be confused with phage F1, a ssDNA phage, but f1 may also refer to a ssDNA phage.}}
== Morphology ==
== Morphology ==
Phage f1 is a filamentous (rod-shaped) ssDNA phage. <ref name='PMID:14065763'/>  The rods are about 8500 Å long, with a weight average molecular weight of 1.1x10<sup>7</sup>.
Phage f1 is a filamentous (rod-shaped) ssDNA phage. <ref name='PMID:14065763'/>  The rods are about 8500 Å long, with a weight average molecular weight of 1.1x10<sup>7</sup>.  Depending on the staining technique, the rods appear to be 43 or 63 Å wide <ref name='PMID:14065763'/> , but are accepted to be about 60 Å <ref name='PMID:21082179'/>.


== Genome Organization ==
== Genome Organization ==

Revision as of 20:41, 27 June 2011

Bacteriophage f1 is closely related to Phage M13

The full sequence is available from NCBI at GenBank: J02448.1

Phage f1, a ssRNA phage, should not be confused with phage F1, a ssDNA phage, but f1 may also refer to a ssDNA phage.

Morphology

Phage f1 is a filamentous (rod-shaped) ssDNA phage. [1] The rods are about 8500 Å long, with a weight average molecular weight of 1.1x107. Depending on the staining technique, the rods appear to be 43 or 63 Å wide [1] , but are accepted to be about 60 Å [2].

Genome Organization

6407 bp of ss-DNA, circular

10 genes encoded; generally referred to by Roman numerals I-X as listed on the f1 Gene page.

Life cycle

Adsorption and Injection

f1 is male specific; it only infects Hfr or F+ strains of E. coli. [1]

DNA replication

Packaging

Lysis

See also

References

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  1. ↑ 1.0 1.1 1.2 ZINDER, ND et al. (1963) F1, A ROD-SHAPED MALE-SPECIFIC BACTERIOPHAGE THAT CONTAINS DNA. Virology 20 638-40 PubMed
  2. ↑ Straus, SK et al. (2011) Consensus structure of Pf1 filamentous bacteriophage from X-ray fibre diffraction and solid-state NMR. Eur. Biophys. J. 40 221-34 PubMed