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| | !align=left |Citation | ||
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'''No author listed''' (1981) Isolation and characterization of amber mutations in gene dnaA of escherichia coli K-12. ''J. Bacteriol.'' '''145''':904-13 | |||
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| | !align=left |Abstract | ||
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Amber mutants with defects in the dnaA gene of Escherichia coli K-12 were isolated after localized mutagenesis of the tna-dnaA region of the chromosome. We isolated 36 mutants defective in the initiation of deoxyribonucleic acid replication as determined by their dependence upon integrative suppression by a P2 sig5 prophage. Three of the 36 mutants were shown to contain amber mutations through the use of a temperature-sensitive amber suppressor. These mutations, which mapped between gyrB and tna, were characterized genetically and biochemically as amber mutations in dnaA. | |||
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!align=left |Links | |||
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7007354 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC217197 PMC217197] | |||
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!align=left |Keywords | |||
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Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Chromosome Mapping; DNA Replication; DNA, Bacterial/genetics; Escherichia coli/genetics; Genes; Genetic Complementation Test; Mutation; Transduction, Genetic | |||
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==Significance {{HelpIcon|Help:Literature}} == | ==Significance {{HelpIcon|Help:Literature}} == | ||
{{LitSignificance}} | {{LitSignificance}} | ||
Revision as of 19:33, 1 September 2013
| Citation |
No author listed (1981) Isolation and characterization of amber mutations in gene dnaA of escherichia coli K-12. J. Bacteriol. 145:904-13 |
|---|---|
| Abstract |
Amber mutants with defects in the dnaA gene of Escherichia coli K-12 were isolated after localized mutagenesis of the tna-dnaA region of the chromosome. We isolated 36 mutants defective in the initiation of deoxyribonucleic acid replication as determined by their dependence upon integrative suppression by a P2 sig5 prophage. Three of the 36 mutants were shown to contain amber mutations through the use of a temperature-sensitive amber suppressor. These mutations, which mapped between gyrB and tna, were characterized genetically and biochemically as amber mutations in dnaA. |
| Links | |
| Keywords |
Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Chromosome Mapping; DNA Replication; DNA, Bacterial/genetics; Escherichia coli/genetics; Genes; Genetic Complementation Test; Mutation; Transduction, Genetic |
| edit table |
Significance
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| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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