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| | !align=left |Citation | ||
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'''No author listed''' (1978) Dihydrofolate reductase: the amino acid sequence of the enzyme from a methotrexate-resistant mutant of Escherichia coli. ''Biochemistry'' '''17''':1328-37 | |||
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| | !align=left |Abstract | ||
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The determination of the amino acid sequence of the enzyme dihydrofolate reductase (5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase, EC 1.5.1.3) from a mutant of Escherichia coli B is described. The 159 residues were positioned by automatic Edman degradation of the whole protein, of the reduced and alkylated cyanogen bromide fragments, and of selected tryptic, chymotryptic, and thermolytic digestion products. An N-bromosuccinimide produced fragment of the largest cyanogen bromide peptide was also used in the sequence determination. | |||
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=350268 PubMed] | |||
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!align=left |Keywords | |||
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Amino Acid Sequence; Cyanogen Bromide; Drug Resistance, Microbial; Escherichia coli/enzymology; Methotrexate/pharmacology; Mutation; Peptide Fragments; Tetrahydrofolate Dehydrogenase | |||
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==Significance {{HelpIcon|Help:Literature}} == | ==Significance {{HelpIcon|Help:Literature}} == | ||
{{LitSignificance}} | {{LitSignificance}} | ||
Revision as of 19:25, 1 September 2013
| Citation |
No author listed (1978) Dihydrofolate reductase: the amino acid sequence of the enzyme from a methotrexate-resistant mutant of Escherichia coli. Biochemistry 17:1328-37 |
|---|---|
| Abstract |
The determination of the amino acid sequence of the enzyme dihydrofolate reductase (5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase, EC 1.5.1.3) from a mutant of Escherichia coli B is described. The 159 residues were positioned by automatic Edman degradation of the whole protein, of the reduced and alkylated cyanogen bromide fragments, and of selected tryptic, chymotryptic, and thermolytic digestion products. An N-bromosuccinimide produced fragment of the largest cyanogen bromide peptide was also used in the sequence determination. |
| Links | |
| Keywords |
Amino Acid Sequence; Cyanogen Bromide; Drug Resistance, Microbial; Escherichia coli/enzymology; Methotrexate/pharmacology; Mutation; Peptide Fragments; Tetrahydrofolate Dehydrogenase |
| edit table |
Significance
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Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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