PMID:337110: Difference between revisions

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<span style = 'font-size: large'> '''Foster, TJ'''  (1977) Insertion of the tetracycline resistance translocation unit Tn10 in the lac operon of Escherichia coli K12.''Mol. Gen. Genet.'' '''154''':305-9</span>
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'''No author listed'''  (1977) Insertion of the tetracycline resistance translocation unit Tn10 in the lac operon of Escherichia coli K12. ''Mol. Gen. Genet.'' '''154''':305-9
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!align=left  |Abstract
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The majority of TN10 insertions in the lacZ gene of Escherichia coli occurred in a small region of the promoter distal part of the gene. The resulting mutations were polar on lacY and reverted to Lac+ at a frequency of 10(-8). None of the revertants were Tcr. Furthermore Lac+ Tcr revertants could not be selected directly. Relief of polarity revertants of the lacZ::Tn10 mutants were formed at a frequency of 10(-5) - 10(-4). Most resulted from a deletion event internal to the transposon which removed the Tcr genes and the putative transcription terminator. It is postulated that a fragment of Tn10 remains at the original insertion point to cause a revertible Lac- mutation.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=337110 PubMed]


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|{{TableHeadingColor}} |'''Abstract''' || <protect>The majority of TN10 insertions in the lacZ gene of Escherichia coli occurred in a small region of the promoter distal part of the gene. The resulting mutations were polar on lacY and reverted to Lac+ at a frequency of 10(-8). None of the revertants were Tcr. Furthermore Lac+ Tcr revertants could not be selected directly. Relief of polarity revertants of the lacZ::Tn10 mutants were formed at a frequency of 10(-5) - 10(-4). Most resulted from a deletion event internal to the transposon which removed the Tcr genes and the putative transcription terminator. It is postulated that a fragment of Tn10 remains at the original insertion point to cause a revertible Lac- mutation.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=337110 PubMed]<br/>
Chromosome Mapping; Chromosomes, Bacterial; Escherichia coli/enzymology; Escherichia coli/genetics; Galactosidases/metabolism; Genes; Lactose/genetics; Mutation; Operon; R Factors; Recombination, Genetic; Tetracycline/pharmacology; Translocation, Genetic
 
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==Significance {{HelpIcon|Help:Literature}} ==
==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}
{{LitSignificance}}

Revision as of 19:24, 1 September 2013

Citation

No author listed (1977) Insertion of the tetracycline resistance translocation unit Tn10 in the lac operon of Escherichia coli K12. Mol. Gen. Genet. 154:305-9

Abstract

The majority of TN10 insertions in the lacZ gene of Escherichia coli occurred in a small region of the promoter distal part of the gene. The resulting mutations were polar on lacY and reverted to Lac+ at a frequency of 10(-8). None of the revertants were Tcr. Furthermore Lac+ Tcr revertants could not be selected directly. Relief of polarity revertants of the lacZ::Tn10 mutants were formed at a frequency of 10(-5) - 10(-4). Most resulted from a deletion event internal to the transposon which removed the Tcr genes and the putative transcription terminator. It is postulated that a fragment of Tn10 remains at the original insertion point to cause a revertible Lac- mutation.

Links

PubMed

Keywords

Chromosome Mapping; Chromosomes, Bacterial; Escherichia coli/enzymology; Escherichia coli/genetics; Galactosidases/metabolism; Genes; Lactose/genetics; Mutation; Operon; R Factors; Recombination, Genetic; Tetracycline/pharmacology; Translocation, Genetic

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